Anti-Topo II alpha (Clone: ABM48B4 )

Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response. Protein function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. [The UniProt Consortium]