Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions: The charge of nucleic acids and proteins is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry buffering is achieved by addition of so called “good buffers” or PBS.

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing... read more »
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Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions: The charge of nucleic acids and proteins is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry buffering is achieved by addition of so called “good buffers” or PBS.

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Bis-Tris, ultra pure
Bis-Tris, ultra pure

Item number: 50003.100

Useful pH range: 5.8-7.2
CAS 6976-37-0
MW: 209.24
82.00€ *
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HEPES, ultra pure
HEPES, ultra pure

Item number: 05288.1

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. It is one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by...
CAS 7365-45-9
MW: 238.31
From 38.00€ *
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MES, ultra pure
MES, ultra pure

Item number: 06010.100

MES (2-(N-morpholino)ethanesulfonic acid) is used as a buffering agent. It has pKa value of 6.15 at 20°C. The pH (and pKa at ionic strength I?0) of the buffer solution changes with concentration and temperature. MES is highly soluble in water. The melting point is approx. 300 °C. MOPS is a similar pH buffering...
CAS 4432-31-9
MW: 195.24
From 61.00€ *
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Tris, ultra pure
Tris, ultra pure

Item number: 08003.1

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It is a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes.
Keywords: Trometamol, Tromethamin, Tris(hydroxymethyl)-aminomethan, 2-Amino-2-(hydroxymethyl)-propan-1,3-diol
CAS 77-86-1
MW: 121.14
From 61.00€ *
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MOPS, sodium salt
MOPS, sodium salt

Item number: 50429.1

Useful pH range: 6.5-7.9
CAS 71119-22-7
MW: 231.25
From 62.00€ *
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HEPES, sodium salt
HEPES, sodium salt

Item number: 50432.1

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. It is one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by...
CAS 75277-39-3
MW: 260.28
From 54.00€ *
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Tricine, ultra pure
Tricine, ultra pure

Item number: 22870.100

Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a higher negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be...
CAS 5704-04-1
MW: 179.17
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MOPS, ultra pure
MOPS, ultra pure

Item number: 06170.1

MOPS is frequently used as a buffering agent. It is a structural analog to MES. Its chemical structure contains a morpholine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
CAS 1132-61-2
MW: 209.26
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Bicine, ultra pure
Bicine, ultra pure

Item number: 01848.500

Bicine is an organic buffering agent. It is one of Good's buffers and has a pKa of 8.35 at 20°C. It is prepared by the reaction of glycine with ethylene oxide, followed by hydrolysis of the resultant lactone. Useful pH range: 7.8-8.8
CAS 150-25-4
MW: 163.17
From 54.00€ *
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PIPES, ultra pure
PIPES, ultra pure

Item number: 52755.100

Useful pH range: 6.1-7.5
CAS 5625-37-6
MW: 302.40
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Tris Hydrochloride (Tris(hydroxymethyl) aminomethane_HCl,_2-Amino-2- (hydroxymethyl)-1, 3-propanedio
Tris Hydrochloride (Tris(hydroxymethyl)...

Item number: T8650.1

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer. One of the biological buffers. Synonyms: Tris(hydroxymethyl)aminomethane HCl, 2-Amino-2-(hydroxymethyl)-1,3-propanediol HCl, Tromethamine HCl, CAS No: C4H11NO3HCl, Molecular Formula: C4H11NO3HCl,...
CAS 1185-53-1
MW: 157.60 D
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MOPS Free Acid (Morpholinopropanesulfonic acid)
MOPS Free Acid (Morpholinopropanesulfonic acid)

Item number: M4610.500

MOPS Free Acid is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 1132-61-2
MW: 209.30 D
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