Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions: The charge of nucleic acids and proteins is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry buffering is achieved by addition of so called “good buffers” or PBS.

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing... read more »
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Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions: The charge of nucleic acids and proteins is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry buffering is achieved by addition of so called “good buffers” or PBS.

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Bicine, ultra pure
Bicine, ultra pure

Item number: 01848.500

Bicine is an organic buffering agent. It is one of Good's buffers and has a pKa of 8.35 at 20°C. It is prepared by the reaction of glycine with ethylene oxide, followed by hydrolysis of the resultant lactone. Useful pH range: 7.8-8.8
CAS 150-25-4
MW: 163.17
53.00€ From 47.70€
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Bis-Tris, ultra pure
Bis-Tris, ultra pure

Item number: 50003.100

Useful pH range: 5.8-7.2
CAS 6976-37-0
MW: 209.24
80.00€ 72.00€
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Tricine, ultra pure
Tricine, ultra pure

Item number: 22870.500

Useful pH range: 7.4-8.8
CAS 5704-04-1
MW: 179.17
45.00€ From 40.50€
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HEPES, ultra pure
HEPES, ultra pure

Item number: 05288.250

Useful pH range 7.0-8.0
CAS 7365-45-9
MW: 238.31
37.00€ From 33.30€
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MES, ultra pure
MES, ultra pure

Item number: 06010.500

Useful pH range: 5.8-6.5
CAS 4432-31-9
MW: 195.24
60.00€ From 54.00€
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MOPS, ultra pure
MOPS, ultra pure

Item number: 06170.500

Useful pH range: 6.5-7.9
CAS 1132-61-2
MW: 209.26
31.00€ From 27.90€
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Tris, ultra pure
Tris, ultra pure

Item number: 08003.500

Purity: >99.9%. pKa(20 °C)=8.3. Useful PH range: 7.5-9.0. Highest purity for use in molecular biology.
CAS 77-86-1
MW: 121.14
60.00€ From 54.00€
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MOPS, sodium salt
MOPS, sodium salt

Item number: 50429.1

Useful pH range: 6.5-7.9
CAS 71119-22-7
MW: 231.25
61.00€ From 54.90€
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HEPES, sodium salt
HEPES, sodium salt

Item number: 50432.100

Useful pH range 7.0-8.0
CAS 75277-39-3
MW: 260.28
53.00€ From 47.70€
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PIPES, ultra pure
PIPES, ultra pure

Item number: 52755.500

Useful pH range: 6.1-7.5
CAS 5625-37-6
MW: 302.40
78.00€ From 70.20€
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TES, ultra pure
TES, ultra pure

Item number: 22080.500

Useful pH range: 7.0-8.0
CAS 7365-44-8
MW: 229.18
115.00€ From 103.50€
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Bis-Tris-Propane, pure
Bis-Tris-Propane, pure

Item number: 50224.500

Useful pH range: 6.3-9.5
CAS 64431-96-5
MW: 282.40
195.00€ From 175.50€
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