Proteins and Enzymes

NF-kappaB p65 is a ubiquitously expressed transcription factor that is a subunit of the NF-kappaB complex and is encoded by the RELA gene in humans. It is composed of an N-terminal Rel homology domain, which mediates dimerization, nuclear localization, and DNA and protein interactions, and two C-terminal...

Methyltransferase-like protein 3 (METTL3) and METTL14 are m6A RNA methyltransferases encoded by the METTL3 and METTL14 genes, respectively, in humans. METTL3 and METTL14 form a stable complex in the cytoplasm then localize to the nucleus via a METTL3 nuclear localization sequence. METTL3 contains an N-terminal...

Glial fibrillary acidic protein (GFAP) is a protein encoded by the GFAP gene in humans and a member of the class III intermediate filament (IF) protein family. It is composed of an N-terminal head domain, a highly conserved alpha-helical rod domain, and a C-terminal tail domain that mediate GFAP self-assembly,...

Histone H4 is one of four core histone proteins, the others being H3, H2B, and H2A, that are involved in the organization of DNA into chromatin. Histones are subject to a variety of posttranslational modifications, such as methylation, acetylation, and citrullination, that can influence chromatin structure and...

Fat mass and obesity-associated (FTO) protein is a nuclear-residing N6-methyladenosine (m6A) RNA demethylase that is encoded by the FTO gene in humans. It is composed of an N-terminal domain similar in structure to members of the AlkB non-heme iron-containing dioxygenase family and a C-terminal domain that is not...

Methyltransferase-like protein 16 (METTL16) is an m6A RNA methyltransferase encoded by the METTL16 gene in humans. It is composed of a methyltransferase domain that associates with RNA and two vertebrate conserved regions (VCRs) on the C-terminal end that promote splicing. METTL16 acts as an epigenetic writer by...

Human NLR Family Pyrin Domain Containing 3 (NLRP3), also known as NALP3, Cold Autoinflammatory Syndrome 1 Protein (CIAS1), cryopyrin and PYPAF1, Genbank Accession No. NM_004895, a.a. 134-1036(end) with N-terminal FLAG-tag, expressed in a HEK293 expression system. MW = 105 kDa.

Human NEK7, also known as NIMA Related Kinase 7, GenBank Accession No. NM_133494, a.a. 2-302 (end), with N-terminal His-tag, expressed in a HEK293 cell expression system. MW=37 kDa.

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues are recognized by a small protein domain known as a bromodomain. These domains function in linking protein complexes to acetylated nucleosomes, thereby controlling chromatin...

Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of...

Bovine Serum Albumin (BSA), Fraction V is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screenining assays, western blotting, and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as...

See also 18-161.