Proteins and Enzymes

Fibrinogen is a plasma clotting factor composed of three different polypeptide chains (alpha, beta and gamma). Under chronic inflammatory conditions, fibrinogen can be acted upon by the enzyme peptidylarginine deiminase 4 (PAD4), converting specific arginine residues to citrulline. Patients with rheumatoid arthritis (RA) produce antibodies that bind citrullinated human...

Ready for use. Recombinant TEV protease with an N-terminal His-tag for simple removal from the cleavage reaction by immobilization on metal-affinity resins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S), with cleavage occuring between Q and G or Q and S. The most commonly used sequence is ENLYFQG. This TEV protease contains a S219V...

Bone morphogenetic proteins (BMP) are secreted signaling proteins, many of which are involved in various developmental processes, in addition to bone formation. GDF11 is a circulating growth and differentiation factor that has age-dependent effects on development. Notably, injection of recombinant GDF11 into old mice, so that circulating levels match those in young mice,...

Ready for use. Recombinant TVMV protease with an N-terminal His-tag for simple removal from the cleavage reaction by immobilization on metal-affinity resins. TVMV protease cleaves the sequence ETVRQS, with cleavage occuring between Q and S residues with high specifity. It is mainly used for removing affinity tags from peptides. Affinity tags are often applied to facilitate...

The heterochromatin protein 1 (HP1) family, which consists of three isoforms HP1alpha (CBX5), HP1beta (CBX1), and HP1gamma (CBX3) are chromatin-associated proteins involved in gene regulation and heterochromatin formation. CBX proteins have an N-terminal chromodomain, a C-terminal chromoshadow domain (CSD), and a hinge domain which connects the two. Extensive...

CBX2 is a component of the polycomb repressive complex 1 and represses protein expression. The chromodomain regions of CBX2 recognizes histone H3 trimethyl lysine 9 (H3K9me3) or trimethyl lysine 27 (H3K27me3). CBX2 knock-out mice have impaired spleen formation, T cell expansion, and inactivation of the X chromosome, which can be lethal in some cases. This protein product...

Key Features Cayman's L3MBTL1 MBT Domains TR-FRET Assay Kit (Cay-601030) was developed using L3MBTL1 MBT domains (human recombinant, GST-tagged) protein.Bulk sizes available upon request "The malignant brain tumor (MBT) domain is structurally related to chromatin binding domains, such as chromodomains, tudor domains, and PWWP-domains. MBT domain-containing proteins have a...

Recognition of epigenetic marks can be mediated by small modular protein units of ~50 amino acids called Plant Homeodomain (PHD) fingers. PHD fingers are zinc binding domains that have a Cys4-His-Cys3 motif and are found in more than 100 nuclear proteins that play a role in regulating chromatin. PHD domains often work with other protein regions, such as bromodomains and...

Tudor domains (or tudor-like regions) are small protein structural motifs of ~50 amino acids related to the "Royal Family" of methyl readers, which also includes chromo, malignant brain tumor (MBT), PWWP, and Agenet-like domains. Tudor domains occur either alone, in tandem, or with other domains and are found in many proteins that are involved in RNA metabolism, germ cell...

HMG-CoA reductase (HMGR) is a highly regulated enzyme found on the endoplasmic reticulum (ER) membrane. It is bound to the ER membrane by a multi-pass 339 amino acid N-terminal transmembrane domain while the carboxy terminal catalytic region projects into the cytosol. HMGR is controlled by feedback regulation from sterols and non-sterol metabolites derived from mevalonate....

Eukaryotic translation is a complex biochemical process requiring several initiation factors. The first step in the initiation of mRNA translation involves binding of the small ribosomal subunit to mRNA. The central component in the recruitment of the pre-initiation complex during translation is the eukaryotic initiation factor 4F (eIF4F) complex, composed of eIF4A, eIF4E,...

Nicotinamide N-Methyltransferase (NNMT) methylates nicotinamide and other pyridine containing compounds. NNMT transfers a methyl group from S-adenosylmethionine (SAM) to nitrogen N1 of nicotinamide to produce 1-methylnicotinamide (MNA) and S-adenosylhomocysteine. MNA is a messenger molecule that increases neurite branching, serves as an anti-thrombotic, and has...