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Item number: RN200.3
Radial Immunodiffusion (RID) is a quantitative immunodiffusion technique used to determine the concentration of an antigen in a biological fluid. This method is based on the diffusion of an antigen in a radial pattern from a cylindrical well through an agarose gel containing an appropriate mono-specific antibody. As the antigen diffuses radially in all directions into the...
Item number: ABD-1230
AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. iFluor(TM) 594 dyes have fluorescence excitation and emission maxima of 590 nm and 610 nm respectively. iFluor(TM) 594 family has the spectral properties similar to those of Texas Red(R) and Alexa Fluor(R) 594 (Texas Red(R) and Alexa Fluor(R) 594 are the trademarks of Invitrogen)....
Item number: BM-HL007R010SG
For easy starting we offer a HOPE® Trail Kit with 100 ml HOPE® I, 1ml HOPE® II and 500 g Low Melting Paraffin sufficient for fixation of 20 tissue samples. HOPE® = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect - is a new formalin-free tissue conservation technique for tissue fixation, including subsequent paraffin-embedding and sectioning that allows...
|Application:||Tissue fixation, IHC, ISH|
Item number: Cay601070-1
Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and...
|Application:||Isolating mouse neutrophils by peritoneal lavage, FC|
Item number: Cay601220-1
Cayman's Nitrotyrosine IP Kit allows for the capture and pulldown of nitrated proteins from cell lysates using a sorbent coupled with a nitrotyrosine monoclonal antibody. The nitrated proteins can be eluted from the resin, loaded to an SDS-PAGE gel, and probed using the included Nitrotyrosine Polyclonal Antibody or a user supplied target specific antibody. A positive control...
|Application:||Electrophoresis, IP, MS, WB|
Item number: Cay601510-100
This kit is designed for the sensitive, accurate detection and quantitation of diene-containing compounds such as Vitamin D or Vitamin D metabolites. This is achieved through chemical derivatization utilizing a Derivatization Reagent based upon click chemistry. Included in this kit is a simple, rapid, and efficient protocol to extract and derivatize diene compounds from...
Item number: Cay701010-1
When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman's MitoCheck(R) Mitochondrial (Tissue) Isolation Kit includes a complete package of...
Item number: ABE-17-1012
The Intracellular FLOW Staining Kit allows detection antibodies to access the intracellular protein in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. First, live cells can be fixed with the Fixation buffer, which cross-links the protein. Second, the 1X...
Item number: ABE-17-1013
The Cell Surface FLOW Staining Kit allows the detection of antibodies on the cellsurface proteins in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. This buffer can be used for cell and antibody dilution steps, also wash steps for the cell surface staining and...
Item number: ABE-17-1014
The FoxP3 / Transcription Factor Staining Kit has optimized for staining with antibodies to transcription factors and nuclear proteins, such as FoxP3. This kit can be used for staining cytokines and chemokines.