Toolkits

When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman's MitoCheck(R) Mitochondrial (Tissue) Isolation Kit includes a complete package of...

For easy starting we offer a HOPE® Trail Kit with 100 ml HOPE® I, 1ml HOPE® II and 500 g Low Melting Paraffin sufficient for fixation of 20 tissue samples. HOPE® = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect - is a new formalin-free tissue conservation technique for tissue fixation, including subsequent paraffin-embedding and sectioning that allows...

Cayman's 8-Isoprostane Affinity Sorbent provides a specific and convenient means for the purification of 8-isoprostane (8-iso PGF2alpha) from urine and plasma samples. Each vial contains 1 ml of affinity sorbent (mouse anti-8-isoprostane monoclonal antibody covalently bound to Sepharose 4).

Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and...

Cayman's Nitrotyrosine IP Kit allows for the capture and pulldown of nitrated proteins from cell lysates using a sorbent coupled with a nitrotyrosine monoclonal antibody. The nitrated proteins can be eluted from the resin, loaded to an SDS-PAGE gel, and probed using the included Nitrotyrosine Polyclonal Antibody or a user supplied target specific antibody. A positive control...

This kit is designed for the sensitive, accurate detection and quantitation of diene-containing compounds such as Vitamin D or Vitamin D metabolites. This is achieved through chemical derivatization utilizing a Derivatization Reagent based upon click chemistry. Included in this kit is a simple, rapid, and efficient protocol to extract and derivatize diene compounds from...

The Intracellular FLOW Staining Kit allows detection antibodies to access the intracellular protein in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. First, live cells can be fixed with the Fixation buffer, which cross-links the protein. Second, the 1X...

The Cell Surface FLOW Staining Kit allows the detection of antibodies on the cellsurface proteins in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. This buffer can be used for cell and antibody dilution steps, also wash steps for the cell surface staining and...

The FoxP3 / Transcription Factor Staining Kit has optimized for staining with antibodies to transcription factors and nuclear proteins, such as FoxP3. This kit can be used for staining cytokines and chemokines.

Costar(R) 96-Well EIA/RIA Stripwell(TM) Plate is recommended for use with our ELISA Solutions in EIA, RIA, and ELISA testing formats. The Costar(R) 1 x 8 strips are pre-assembled in a standard 96-well plate holder (white). The individual 8-well strips are designed to fit only one way into the holder, assuring proper orientation. Sold individually.