Epitope Tag Antibodies

GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against...

Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection...

Protein function: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. [The UniProt Consortium]

WB:1:10000, ELISA: 1:15000-150000, IHC, IP:1:250. The antibody is useful in determining the HA motif in various assays. Recommended secondary ab: 611-703-127. HA epitope tag sequence: Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala

WB: 0.5-1.0 µg/ml, ELISA: 1:10.000. The antibody recognizes the His tag fused either to the amino- or carboxy-termini of targeted proteins.

Protein function: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. [The UniProt Consortium]

0.1 mg antibody/0.4 ml of 50% gel slurry

Mouse anti-His tag mAb is specific to 6X His tag which can be applied to several immunoassays including ELISA, WB, IP, IFA and FACS. This antibody has been identified in several sources (bacteria, yeast and mammalian cells) of His tag fusion protein.

Mouse anti-cMyc tag mAb is specific to tags which can be applied to several immunoassays including ELISA, WB, IP, IFA and FACS. This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of cMyc fusion protein.

Mouse anti-V5 tag mAb is specific to GKPIPNPLLG (V5) tag which can be applied to several immunoassays including ELISA, WB, IP, IFA and FACS. This antibody has been identified in several sources (bacteria, yeast and mammalian cells) of V5 tag fusion protein.