ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of... read more »
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ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

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Thromboxane B2 ELISA Kit
Thromboxane B2 ELISA Kit

Item number: Cay501020-96

Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short distance of its biosynthesis. TXA2 is rapidly hydrolyzed...
Application: ELISA
Format: Solid Phase
From 357.00€
Review
6-keto Prostaglandin F1alpha ELISA Kit
6-keto Prostaglandin F1alpha ELISA Kit

Item number: Cay515211-96

Prostacyclin (Prostaglandin I2, PGI2) is formed from arachidonic acid primarily by the vascular endothelium and renal cortex. It is a potent vasodilator and inhibitor of platelet aggregation. PGI2 is non-enzymatically hydrated to 6-keto PGF1alpha (t½ = 2-3 minutes), and then quickly converted to the major metabolite, 2,3-dinor-6-keto PGF1alpha (t½ = 30 minutes). Prostacyclin...
Application: ELISA
Format: Solid Phase
From 338.00€
Review
2'3'-cGAMP ELISA Kit
2'3'-cGAMP ELISA Kit

Item number: Cay501700-96

Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that detects the presence of nucleic acids in the cytosol of mammalian cells as an indicator of bacterial or viral infection. cGAS catalyzes the synthesis of a second messenger, 2'3'-cGAMP, from cytosolic ATP and GTP in response to dsDNA binding. 2'3'-cGAMP then binds tightly to the adaptor protein STING (stimulator of...
Application: ELISA
Format: Solid Phase
494.00€
Review
Obestatin (human) EIA Kit
Obestatin (human) EIA Kit

Item number: Cay18902-96

Obestatin is an amidated peptide of 23 amino-acids with an alpha-helical secondary conformation. It was first isolated from rat stomach in 2005. Obestatin is a preproghrelin-derived peptide produced by many tissues or organs, including the stomach, pancreas, adipose tissue, skeletal muscle, and heart. Obestatin was originally identified as an anorexigenic peptide that...
Application: ELISA
Reactivity: Human
Format: Solid Phase
562.00€
Review
Obestatin (mouse, rat) EIA Kit
Obestatin (mouse, rat) EIA Kit

Item number: Cay18903-96

Obestatin is an amidated peptide of 23 amino acids with an alpha-helical secondary conformation. It was first isolated from rat stomach in 2005. Obestatin is a preproghrelin-derived peptide produced by many tissues or organs, including the stomach, pancreas, adipose tissue, skeletal muscle, and the heart. Obestatin was originally identified as an anorexigenic peptide that...
Application: ELISA
Reactivity: Mouse, Rat
Format: Solid Phase
562.00€
Review
Host Cell Proteins - HCP (E. coli) EIA Kit
Host Cell Proteins - HCP (E. coli) EIA Kit

Item number: Cay18919-96

Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as 'Host Cell Proteins (HCP)', are generated and need to be removed. This kit is intended for use in assessing relative quantities of E. coli HCP in manufactured or...
Application: ELISA, HTS
Reactivity: E.coli
Format: Solid Phase
600.00€
Review
Acylated Ghrelin (pig) ELISA Kit
Acylated Ghrelin (pig) ELISA Kit

Item number: Cay23270-96

Bertin Pharma's expertise is to develop analytical tools for biomarkers. As such, in 2004, Bertin Pharma launched its first Ghrelin Biomarker assay kits, under the SPI-Bio brand name, as a result of its state-of-the-art R&D research teams. Bertin Pharma was the first company providing bioanalytical tools to assay Acylated and Non-acylated Ghrelin with very high sensitivity....
Application: ELISA
Reactivity: Swine
Format: Solid Phase
625.00€
Review
Unacylated Ghrelin (pig) ELISA Kit
Unacylated Ghrelin (pig) ELISA Kit

Item number: Cay23271-96

Bertin Pharma's expertise is to develop analytical tools for biomarkers. As such, in 2004, Bertin Pharma launched its first Ghrelin Biomarker assay kits, under the SPI-Bio brand name, as a result of its state-of-the-art R&D research teams. Bertin Pharma was the first company providing bioanalytical tools to assay Acylated and Non-acylated Ghrelin with very high sensitivity....
Application: ELISA
Reactivity: Swine
Format: Solid Phase
625.00€
Review
IL-6 (pig) ELISA Kit
IL-6 (pig) ELISA Kit

Item number: Cay23615-96

Intetleukin-6 (IL-6) is a protein of 212 amino acids. It's a pleiotropic cytokine which has an impact on the regulation of immune and nonimmune events. It is produced by the monocytes and macrophages in response to other inflammatory cytokines like IL-11 and the tumor necrosis factor-beta (TNF-beta). IL-6 acts on immune and nonimmune cells: It helps to differentiate T cells...
Application: ELISA
Reactivity: Swine
Format: Solid Phase
519.00€
Review
TNF-alpha (pig) ELISA Kit
TNF-alpha (pig) ELISA Kit

Item number: Cay23616-96

Known as cachectin, the TNF-alpha is a glycoprotein of 156 amino acids produced from a precursor processed by action of a metalloprotease. Isolated for the first time in 1975 by Carswell, it's a cytokine of the innate immunity. Interleukin-1 or bacterial endotoxins stimulate the production of TNF-alpha by immune cells (activated macrophages, NK cells, and monocytes) and...
Application: ELISA
Reactivity: Swine
Format: Solid Phase
519.00€
Review
IFN-alpha 1 (pig) ELISA Kit
IFN-alpha 1 (pig) ELISA Kit

Item number: Cay23618-96

The IFN-alpha is one of the member of the type I interferons (IFNs) family and it is mainly involved in innate immunity. IFN-alpha 1 is secreted by immune (lymphocytes, NK cells, B cells, T cells, and macrophages) and non-immune cells (fibroblasts, endothelial cells, osteoblasts, and others) in answer to a viral infection. The main function of the IFN-alpha 1 is to alert the...
Application: ELISA
Reactivity: Swine
Format: Solid Phase
519.00€
Review
IFN-gamma (pig) ELISA Kit
IFN-gamma (pig) ELISA Kit

Item number: Cay23619-96

Also known as type II interferons, IFN-gamma is a glycoprotein of 146 amino acids. IFN-gamma is a cytokine critical for the innate and adaptive immunity. It is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response and by T helper cells and cytotoxic T lymphocyte (CTL) as part of the adaptive immunity. IFN-gamma...
Application: ELISA
Reactivity: Swine
Format: Solid Phase
519.00€
Review
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