ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of... read more »
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ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

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Anti-Ovalbumin IgE (mouse) ELISA Kit
Anti-Ovalbumin IgE (mouse) ELISA Kit

Item number: Cay500840-96

Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate tissue-specific effector functions in multiple mouse models of chronic...
Application: ELISA
369.00€
Review
Thromboxane B2 ELISA Kit
Thromboxane B2 ELISA Kit

Item number: Cay501020-96

Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short distance of its biosynthesis. TXA2 is rapidly hydrolyzed...
Application: ELISA
Format: Solid Phase
From 357.00€
Review
Prostaglandin E2 ELISA Kit - Monoclonal
Prostaglandin E2 ELISA Kit - Monoclonal

Item number: Cay514010-96S

Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo, PGE2 is rapidly converted to an inactive metabolite...
Application: ELISA
Format: Solid Phase
From 344.00€
Review
Prostaglandin F2alpha ELISA Kit
Prostaglandin F2alpha ELISA Kit

Item number: Cay516011-96S

Prostaglandin F2alpha (PGF2alpha) is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. It was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2alpha is a potent luteolytic agent and is used to induce ovulation in domestic livestock....
Application: ELISA
Format: Solid Phase
From 357.00€
Review
Fall/Winter ELISA Promotion
11-keto Testosterone ELISA Kit
11-keto Testosterone ELISA Kit

Item number: Cay582751-96

While testosterone is the primary androgenic male steroid found in mammals, 11-keto testosterone (11-KT) is a second key androgenic steroid found in fish. It occurs in males together with testosterone in amounts which vary from less than 1 ng/ml to as much as 50-100 ng/ml, depending on the species and the stage of the reproductive cycle. In the sea bass, testosterone...
Application: ELISA
Format: Solid Phase
369.00€ From 332.10€
Review
DNA/RNA Oxidative Damage ELISA Kit
DNA/RNA Oxidative Damage ELISA Kit

Item number: Cay589320-96

Both DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. The repair processes that are initiated to correct this damage release multiple oxidized guanine species into the urine. Cayman Chemical has developed this immunoassay for the measurement of DNA/RNA oxidative damage that detects all three oxidized guanine species,...
Application: ELISA
Format: Solid Phase
From 619.00€
Review
FREE Swag Bag
VC (Vitamin C) ELISA Kit
VC (Vitamin C) ELISA Kit

Item number: E-EL-0011.96

The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of VC in serum, plasma and other biological fluids. Detection Range: 1.56--100µg/mL. Sensitivity: 0.94µg/mL.
Application: ELISA
Reactivity: Broad
Format: Solid Phase
455.00€
Review
Obestatin (human) EIA Kit
Obestatin (human) EIA Kit

Item number: Cay18902-96

Obestatin is an amidated peptide of 23 amino-acids with an alpha-helical secondary conformation. It was first isolated from rat stomach in 2005. Obestatin is a preproghrelin-derived peptide produced by many tissues or organs, including the stomach, pancreas, adipose tissue, skeletal muscle, and heart. Obestatin was originally identified as an anorexigenic peptide that...
Application: ELISA
Reactivity: Human
Format: Solid Phase
603.00€
Review
Obestatin (mouse, rat) EIA Kit
Obestatin (mouse, rat) EIA Kit

Item number: Cay18903-96

Obestatin is an amidated peptide of 23 amino acids with an alpha-helical secondary conformation. It was first isolated from rat stomach in 2005. Obestatin is a preproghrelin-derived peptide produced by many tissues or organs, including the stomach, pancreas, adipose tissue, skeletal muscle, and the heart. Obestatin was originally identified as an anorexigenic peptide that...
Application: ELISA
Reactivity: Mouse, Rat
Format: Solid Phase
603.00€
Review
Host Cell Proteins - HCP (E. coli) EIA Kit
Host Cell Proteins - HCP (E. coli) EIA Kit

Item number: Cay18919-96

Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as 'Host Cell Proteins (HCP)', are generated and need to be removed. This kit is intended for use in assessing relative quantities of E. coli HCP in manufactured or...
Application: ELISA, HTS
Reactivity: E.coli
Format: Solid Phase
645.00€
Review
Acylated Ghrelin (pig) ELISA Kit
Acylated Ghrelin (pig) ELISA Kit

Item number: Cay23270-96

Bertin Pharma's expertise is to develop analytical tools for biomarkers. As such, in 2004, Bertin Pharma launched its first Ghrelin Biomarker assay kits, under the SPI-Bio brand name, as a result of its state-of-the-art R&D research teams. Bertin Pharma was the first company providing bioanalytical tools to assay Acylated and Non-acylated Ghrelin with very high sensitivity....
Application: ELISA
Reactivity: Swine
Format: Solid Phase
657.00€
Review
Unacylated Ghrelin (pig) ELISA Kit
Unacylated Ghrelin (pig) ELISA Kit

Item number: Cay23271-96

Bertin Pharma's expertise is to develop analytical tools for biomarkers. As such, in 2004, Bertin Pharma launched its first Ghrelin Biomarker assay kits, under the SPI-Bio brand name, as a result of its state-of-the-art R&D research teams. Bertin Pharma was the first company providing bioanalytical tools to assay Acylated and Non-acylated Ghrelin with very high sensitivity....
Application: ELISA
Reactivity: Swine
Format: Solid Phase
657.00€
Review
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