ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of... read more »
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ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

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Testosterone ELISA Kit
Testosterone ELISA Kit

Item number: Cay582701-96

Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis and at puberty....
Keywords: Testosterone EIA Kit
Application: ELISA
From 288.00€ *
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8-Isoprostane ELISA Kit
8-Isoprostane ELISA Kit

Item number: Cay516351-96

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the...
Keywords: 8-epi Prostaglandin F2alpha, 8-iso Prostaglandin F2alpha, 8-Isoprostane EIA Kit, iPF2alpha-III
Application: ELISA
From 338.00€ *
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Prostaglandin E2 ELISA Kit - Monoclonal
Prostaglandin E2 ELISA Kit - Monoclonal

Item number: Cay514010-96

Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo,...
Keywords: PGE2 EIA Kit - Monoclonal
Application: ELISA
From 344.00€ *
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Citrullinated Histone H3 (Clone 11D3) ELISA Kit
Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Item number: Cay501620-96

Histone H3 is citrullinated by peptidylarginine deiminase 4 (PAD4) at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and neutrophil extracellular trap (NET) formation. In vitro, citrullinated H3 (CitH3) is released from neutrophils that have been stimulated with calcium ionophore...
Keywords: CitH3 (Clone 11D3) EIA Kit
Application: ELISA
619.00€ *
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Human Therapeutic IgG1 ELISA Kit
Human Therapeutic IgG1 ELISA Kit

Item number: Cay500910-96

Human therapeutic antibodies have become increasingly common components of early drug discovery and development portfolios in the pharmaceutical and biotech industries. As part of preclinical toxicology assessment of these agents, they are routinely tested in non-human primates, primarily in rhesus or cynomolgus...
Keywords: Human IgG1 Immunometric EIA Kit
Application: ELISA
719.00€ *
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DNA/RNA Oxidative Damage ELISA Kit
DNA/RNA Oxidative Damage ELISA Kit

Item number: Cay589320-96

DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG),...
Keywords: 8-hydroxy-2'-Deoxyguanosine, 8-OH-2dG, 8-OH-DG, DNA/RNA Oxidative Damage (High Sensitivity) EIA Kit
Application: ELISA
From 619.00€ *
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Cyclic AMP ELISA Kit
Cyclic AMP ELISA Kit

Item number: Cay581001-96

Cayman's cAMP assay is a very sensitive competitive ELISA that permits cAMP measurements within the standard curve range of 0.078-10 pmol/ml, typically with a sensitivity (80% B/B0) of 0.1 pmol/ml once samples and standards have been acetylated prior to analysis. This assay has been validated with culture media,...
Keywords: Adenosine 3',5'-cyclic mononucleotide, Adenosine 3',5'-cyclic monophosphate, cAMP EIA Kit
Application: ELISA
From 338.00€ *
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Prostaglandin F2alpha ELISA Kit
Prostaglandin F2alpha ELISA Kit

Item number: Cay516011-96

Prostaglandin F2alpha (PGF2alpha) is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. It was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2alpha is a potent luteolytic...
Keywords: PGF2alpha EIA Kit
Application: ELISA
From 357.00€ *
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Prostaglandin E2 Express ELISA Kit
Prostaglandin E2 Express ELISA Kit

Item number: Cay500141-96

PGE2 is one of the primary COX products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. Because of its involvement in inflammation, PGE2 is the key indicator of non-steroidal...
Keywords: PGE2 Express EIA Kit
Application: ELISA
From 357.00€ *
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Thromboxane B2 ELISA Kit
Thromboxane B2 ELISA Kit

Item number: Cay501020-96

Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short...
Keywords: TXB2 EIA Kit
Application: ELISA
From 357.00€ *
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Cyclic GMP ELISA Kit
Cyclic GMP ELISA Kit

Item number: Cay581021-96

The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was...
Keywords: cGMP EIA Kit, Guanosine 3',5'-cyclic mononucleotide, Guanosine 3',5'-cyclic monophosphate, Monosodium-GMP
Application: ELISA
From 338.00€ *
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Prostaglandin E Metabolite ELISA Kit
Prostaglandin E Metabolite ELISA Kit

Item number: Cay514531-96

Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE2...
Keywords: PGE Metabolite EIA Kit, PGEM
Application: ELISA
From 344.00€ *
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