A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.
A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such...
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A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.