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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.

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Chick Embryo Extract, Ultrafiltrate
Chick Embryo Extract, Ultrafiltrate

Item number: C3999.20

Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an...
From 343.00€ *
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12-Epitope Tag Protein Marker Lysate
12-Epitope Tag Protein Marker Lysate

Item number: MB-301-0100

This product is designed for use as a positive control for anti-epitope tag antibodies.
Application: Posive control
168.00€ *
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Cell lysate from untreated HeLa cells (HeLa/Co)
Cell lysate from untreated HeLa cells (HeLa/Co)

Item number: 0821

Application: WB
88.00€ *
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HeLa Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
HeLa Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X...

Item number: W09-000-364

Application: Positive control
156.00€ *
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Guinea Pig Liver Acetone Tissue Powder
Guinea Pig Liver Acetone Tissue Powder

Item number: C103

361.00€ *
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Jurkat Whole Cell Lysate
Jurkat Whole Cell Lysate

Item number: W09-001-370

Jurkat Whole Cell Lysate 1.0 mg/ml by BCA assay in 1X SDS-PAGE Sample Buffer.
Application: Control
156.00€ *
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NIH/3T3 Whole Cell Lysate
NIH/3T3 Whole Cell Lysate

Item number: W10-000-358

NIH/3T3 Whole Cell Lysate 1.0 mg/ml by BCA assay in 1X SDS-PAGE Sample Buffer.
Application: Control
156.00€ *
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Normal Mouse Pancreas Whole Cell Lysate
Normal Mouse Pancreas Whole Cell Lysate

Item number: W10-000-T023

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
156.00€ *
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HeLa Cell Lysate, Heat Shocked
HeLa Cell Lysate, Heat Shocked

Item number: H1838-01.200

HeLa Cell Lysate (Heat Shocked) is useful in immunoblotting applications as a positive control for many antibodies, particularly those recognizing proteins induced by heat shock. The cells are grown, heat shocked for 2 hours at 44°C, and allowed to recover at 37°C for 5 to18 hours before harvest. The cells are then...
Application: WB
319.00€ *
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HeLa Whole Cell Lysate MG-132 Treated
HeLa Whole Cell Lysate MG-132 Treated

Item number: W09-001-GV9

HeLa cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were treated O/N with 0.2µg/mL of MG-132 in order to reduce degradation of ubiquitin-conjugated proteins by the proteasome. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium...
Application: WB
Reactivity: Human
230.00€ *
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HEK293 Whole Cell Lysate MG-132 Treated
HEK293 Whole Cell Lysate MG-132 Treated

Item number: W09-001-GV8

HEK293 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were treated O/N with 0.2µg/mL of MG-132 in order to reduce degradation of ubiquitin-conjugated proteins by the proteasome. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM...
Application: WB
Reactivity: Human
230.00€ *
Review
Rabbit Liver Acetone Tissue Powder
Rabbit Liver Acetone Tissue Powder

Item number: C108

256.00€ *
Review
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