Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.

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Chick Embryo Extract, Ultrafiltrate
Chick Embryo Extract, Ultrafiltrate

Item number: C3999.100

Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an...
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HeLa Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
HeLa Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X...

Item number: W09-000-364

Application: Positive control
164.00€ *
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12-Epitope Tag Protein Marker Lysate
12-Epitope Tag Protein Marker Lysate

Item number: MB-301-0100

This product is designed for use as a positive control for anti-epitope tag antibodies.
Application: Posive control
176.00€ *
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PC-3 Whole Cell Lysate
PC-3 Whole Cell Lysate

Item number: W09-001-GV6

PC-3 cells were grown in F-12K Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse...
Application: WB
Reactivity: Human
161.00€ *
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U-87 MG Whole Cell Lysate
U-87 MG Whole Cell Lysate

Item number: W09-001-GX2

U-87 MG cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01%...
Application: WB
Reactivity: Human
161.00€ *
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U-251 Whole Cell Lysate
U-251 Whole Cell Lysate

Item number: W09-001-GY4

U-251 cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v)...
Application: WB
Reactivity: Human
161.00€ *
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U-138 MG Whole Cell Lysate
U-138 MG Whole Cell Lysate

Item number: W09-001-MP5

The U-138 MG cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium...
Application: WB
Reactivity: Human
161.00€ *
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Cell lysate from untreated HeLa cells (HeLa/Co)
Cell lysate from untreated HeLa cells (HeLa/Co)

Item number: 0821

Application: WB
88.00€ *
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KG-1 Whole Cell Lysate
KG-1 Whole Cell Lysate

Item number: W09-001-GY6

Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot...
Application: WB
Reactivity: Human
161.00€ *
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Daudi Whole Cell Lysate
Daudi Whole Cell Lysate

Item number: W09-001-MQ2

Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot...
Application: WB
Reactivity: Human
161.00€ *
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Mouse Colon, Adult, Whole Cell Lysate
Mouse Colon, Adult, Whole Cell Lysate

Item number: W10-000-GZ1

Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot...
Application: WB
Reactivity: Mouse
161.00€ *
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Rat brain, post natal, Whole Cell Lysate
Rat brain, post natal, Whole Cell Lysate

Item number: W12-001-MQ6

Multi-purpose Whole Cell Lysates produced by Rockland Immunochemicals are derived from tissues and cell lines using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels. Tissues were...
Application: ELISA, IP, WB
Reactivity: Rat
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