Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA or proteins. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs and allows an evaluation of the antibody’s target specificity.

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Chick Embryo Extract, Ultrafiltrate
Chick Embryo Extract, Ultrafiltrate

Item number: C3999.20

Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an...
From 343.00€ *
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PC-3 Whole Cell Lysate
PC-3 Whole Cell Lysate

Item number: W09-001-GV6

PC-3 cells were grown in F-12K Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse...
Application: WB
Reactivity: Human
164.00€ *
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U-87 MG Whole Cell Lysate
U-87 MG Whole Cell Lysate

Item number: W09-001-GX2

U-87 MG cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01%...
Application: WB
Reactivity: Human
164.00€ *
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U-251 Whole Cell Lysate
U-251 Whole Cell Lysate

Item number: W09-001-GY4

U-251 cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v)...
Application: WB
Reactivity: Human
164.00€ *
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U-138 MG Whole Cell Lysate
U-138 MG Whole Cell Lysate

Item number: W09-001-MP5

The U-138 MG cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium...
Application: WB
Reactivity: Human
164.00€ *
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HeLa Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
HeLa Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X...

Item number: W09-000-364

Application: WB, Positive control
164.00€ *
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12-Epitope Tag Protein Marker Lysate
12-Epitope Tag Protein Marker Lysate

Item number: MB-301-0100

This product is designed for use as a positive control for anti-epitope tag antibodies.
Application: WB, Posive control
176.00€ *
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Guinea Pig Liver Acetone Tissue Powder
Guinea Pig Liver Acetone Tissue Powder

Item number: C103

379.00€ *
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CHO/K1 Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
CHO/K1 Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X...

Item number: W07-000-359

Application: WB, Positive control
164.00€ *
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Jurkat Whole Cell Lysate
Jurkat Whole Cell Lysate

Item number: W09-001-370

Jurkat Whole Cell Lysate 1.0 mg/ml by BCA assay in 1X SDS-PAGE Sample Buffer.
Application: WB, Control
164.00€ *
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HeLa Whole Cell Lysate - (Etoposide Stimulated, 1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffe
HeLa Whole Cell Lysate - (Etoposide Stimulated, 1.0 mg/ml...

Item number: W09-001-A80

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
167.00€ *
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NIH/3T3 Whole Cell Lysate
NIH/3T3 Whole Cell Lysate

Item number: W10-000-358

NIH/3T3 Whole Cell Lysate 1.0 mg/ml by BCA assay in 1X SDS-PAGE Sample Buffer.
Application: WB, Control
164.00€ *
Review
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