Plasmids

Plasmids are small, circular, double-stranded DNA molecules of mostly bacterial origin. A plasmid is often present episomally in the cell, which means that it replicates independently of the cell's genome. This usually results in a high plasmid copy number. Plasmids are primarily used as cloning vectors for the amplification and expression of recombinant DNA. Moreover, plasmids can also be utilized as expression vectors for the biotechnological production of proteins in cell culture. Many plasmids carrying cDNA fragments are commercially available for molecular cloning and plasmids as well as backbones optimized for various expression systems and species have been developed.

Plasmids are small, circular, double-stranded DNA molecules of mostly bacterial origin. A plasmid is often present episomally in the cell, which means that it replicates... read more »
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Plasmids

Plasmids are small, circular, double-stranded DNA molecules of mostly bacterial origin. A plasmid is often present episomally in the cell, which means that it replicates independently of the cell's genome. This usually results in a high plasmid copy number. Plasmids are primarily used as cloning vectors for the amplification and expression of recombinant DNA. Moreover, plasmids can also be utilized as expression vectors for the biotechnological production of proteins in cell culture. Many plasmids carrying cDNA fragments are commercially available for molecular cloning and plasmids as well as backbones optimized for various expression systems and species have been developed.

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EZ Cap(TM) mCherry mRNA (5mCTP, psiUTP)
EZ Cap(TM) mCherry mRNA (5mCTP, psiUTP)

Item number: Cay39799-1

EZ Cap(TM) mCherry mRNA encodes for mCherry, a red fluorescent protein and derivative of Discosoma red fluorescent protein (DsRed) that displays excitation and emission maxima of 587 and 610 nm, respectively. It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure...
Application: MCherry mRNA expression
From 377.00€ *
Review
EZ Cap(TM) EGFP mRNA (mo5U)
EZ Cap(TM) EGFP mRNA (mo5U)

Item number: Cay39800-1

EZ Cap(TM) EGFP mRNA encodes for enhanced GFP (EGFP), a variant of A. victoria GFP that contains chromophore mutations that increase the sensitivity of the protein and displays excitation and emission maxima of 489 and 511 nm, respectively. It is capped using a co-transcriptional capping method, resulting in the...
Keywords: EZ Cap(TM) EGFP mRNA (5-moUTP)
Application: EGFP mRNA expression
From 369.00€ *
Review
EZ Cap(TM) Firefly Luciferase mRNA (mo5U)
EZ Cap(TM) Firefly Luciferase mRNA (mo5U)

Item number: Cay39801-1

EZ Cap(TM) Firefly Luciferase mRNA encodes for luciferase, a protein originally isolated from the firefly P. pyralis, that catalyzes the ATP-dependent oxidation of the molecule luciferin resulting in chemiluminescence at a wavelength of approximately 560 nm. It is capped using a co-transcriptional capping method,...
Keywords: EZ Cap(TM) Firefly Luciferase mRNA (5-moUTP)
Application: Luciferase mRNA expression
From 613.00€ *
Review
EZ Cap(TM) Cre mRNA (PsiUTP)
EZ Cap(TM) Cre mRNA (PsiUTP)

Item number: Cay39802-1

EZ Cap(TM) Cre mRNA encodes for Cre, a bacteriophage tyrosine recombinase that catalyzes homologous recombination between two loxP sites in DNA. It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. EZ Cap(TM) Cre mRNA is also...
Application: Cre mRNA expression
From 349.00€ *
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DNA Plasmid pUC19, BioAssay
DNA Plasmid pUC19, BioAssay

Item number: D3932-11.50

pUC19 DNA is isolated from E. coli (dam+, dcm+). The molecule is a double-stranded closed circular high copy plasmid, 2686 base pairs in length. GenBank/EMBL accession number L09137. pUC19 is identical to pUC18 except that the polylinker is inserted in the opposite orientation. pUC19 DNA has a molecular weight of...
356.00€ *
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Baculovirus Expression Vector pAc-k-CH3
Baculovirus Expression Vector pAc-k-CH3

Item number: E9073-50.5

Baculovirus cassette vector pAc-k-CH3 for the expression of human, humanized or chimeric IgG(kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fv antibody gene fragments. Vector Size: 7363bp, Resistance:...
1,060.00€ *
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Baculovirus Expression Vector pAc-k-Fc
Baculovirus Expression Vector pAc-k-Fc

Item number: E9073-52.5

Baculovirus cassette vector pAc-k-Fc for the expression of human, humanized or chimeric IgG(kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fab antibody gene fragments. Within the past decade,...
1,060.00€ *
Review
TCF Reporter Plasmid (TOPflash, TK-luciferase reporter), Affinity Purified
TCF Reporter Plasmid (TOPflash, TK-luciferase reporter),...

Item number: T2030.5

Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash (F5950) containing mutated TCF binding...
1,356.00€ *
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ANPRA/Aequorin Expression vector
ANPRA/Aequorin Expression vector

Item number: BPS-60641

The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells. Application: Suitable for use with the PDE1B Cell-based Reporter Assay Kit,...
Keywords: ANPRA, natriuretic peptide receptor A, guanylate cyclase A, jellyfish aequorin,
Application: PDE1B cell-based reporter assays
Species reactivity: human
1,317.00€ *
Review
CNGA2 Expression vector
CNGA2 Expression vector

Item number: BPS-60642

The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells. Application: Suitable for use with the PDE1B Cell-based Reporter Assay Kit, Cat. #BPS- 60601.Storage buffer: 10 mM Tris-HCl (pH 8), 1 mM EDTAStorage condition: Store plasmid at -20°C in...
Keywords: cyclic nucleotide-gated cation channel 2, CNGA2,
Application: PDE1B cell-based reporter assays
Species reactivity: human
1,317.00€ *
Review
COX-1, promoter construct,  Murine (Cyclooxygenase-1,  PGHS-1, Prostaglandin  Endoperoxide Synthase-
COX-1, promoter construct, Murine (Cyclooxygenase-1,...

Item number: C7904-72B.50

The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The...
1,121.00€ *
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DNA, ds (Plasmid), Highly Purified
DNA, ds (Plasmid), Highly Purified

Item number: D4015-06.250

The dsDNA antigen is a 2690bp plasmid purified by alkaline lysis and chromatography. The purification process includes operations to minimize reactivity to antibodies against single-stranded DNA. Use of plasmid DNA in ELISA is an effective method for the detection of anti-double-stranded (ds) DNA antibodies (1). The...
Application: ELISA
1,212.00€ *
Review
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