Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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GAPD, Human GAPDH, Real Time PCR Primer Set
GAPD, Human GAPDH, Real Time PCR Primer Set

Item number: VHPS-3541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: GAPD, GAPDH, CDABP0047, EC=1.2.1.12, EC=2.6.99.-, Peptidyl-cysteine S-nitrosylase GAPDH, Glyceraldehyde-3-phosphate...
Application: RNA quantification
43.00€ *
Review
STI3 (BioRad) Multiplexed qPCR Assay
STI3 (BioRad) Multiplexed qPCR Assay

Item number: Cay40686-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Virus dsDNA, Multiplexed...
Keywords: Sexually transmitted infection detection: C. trachomatis, N. gonorrhoeae, T. pallidum, herpes simplex virus 1 and 2, human...
Application: Sexually transmitted infection detection
735.00€ *
Review
STI4 (Bio-Rad) Multiplexed qPCR Assay
STI4 (Bio-Rad) Multiplexed qPCR Assay

Item number: Cay40687-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Virus dsDNA, Multiplexed...
Keywords: Sexually transmitted infection detection: C. trachomatis, N. gonorrhoeae, HSV1, HSV2, human RPP30 DNA detection
Application: Sexually transmitted infection detection
735.00€ *
Review
STI5 (most common STI) (Bio-Rad) Multiplexed qPCR Assay
STI5 (most common STI) (Bio-Rad) Multiplexed qPCR Assay

Item number: Cay40688-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Protozoan, Multiplexed...
Keywords: Sexually transmitted infection detection: C. trachomatis, N. gonorrhoeae, T. pallidum, T. vaginalis, human RPP30-DNA...
Application: Sexually transmitted infection detection
735.00€ *
Review
STI6 (Lesions) (Bio-Rad) Multiplexed qPCR Assay
STI6 (Lesions) (Bio-Rad) Multiplexed qPCR Assay

Item number: Cay40689-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Virus dsDNA, Multiplexed...
Keywords: Sexually transmitted infection detection: HSV-1, HSV-2, T. pallidum, Monkeypox, human RPP30-DNA detection
Application: Sexually transmitted infection detection
735.00€ *
Review
STI7 (general STI often found together) (Bio-Rad) Multiplexed qPCR Assay
STI7 (general STI often found together) (Bio-Rad)...

Item number: Cay40690-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria (no cell wall), Bacteria Gram-,...
Keywords: Sexually transmitted infection detection: C. trachomatis, N. gonorrhoeae, M. genitalium, T. vaginalis, human RPP30-DNA...
Application: Sexually transmitted infection detection
735.00€ *
Review
STI8 (Genital ulcer) (Bio-Rad) Multiplexed qPCR Assay
STI8 (Genital ulcer) (Bio-Rad) Multiplexed qPCR Assay

Item number: Cay40691-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Virus dsNDA, Multiplexed...
Keywords: Sexually transmitted infection detection: H. ducreyi, C. trachomatis, HSV-2, T. pallidum, human RPP30-DNA detection
Application: Sexually transmitted infection detection
735.00€ *
Review
STI9 4-color (Bio-Rad) Multiplexed qPCR Assay
STI9 4-color (Bio-Rad) Multiplexed qPCR Assay

Item number: Cay40692-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme...
Keywords: Sexually transmitted infection detection: C. trachomatis, HSV-2, M. genitalium, human RPP30-DNA detection
Application: Sexually transmitted infection detection
669.00€ *
Review
STI9 4-color (QuantStudio) Multiplexed qPCR Assay
STI9 4-color (QuantStudio) Multiplexed qPCR Assay

Item number: Cay40693-1

Oliogonucleotide recipes are optimized for QuantStudio 5, 6, 7, 12K. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme...
Keywords: Sexually transmitted infection detection: C. trachomatis, HSV-2, M. genitalium, human RPP30-DNA detection
Application: Sexually transmitted infection detection
669.00€ *
Review
STI9 4-color (MIC) Multiplexed qPCR Assay
STI9 4-color (MIC) Multiplexed qPCR Assay

Item number: Cay40694-1

Oliogonucleotide recipes are optimized for BIS MIC. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism...
Keywords: Sexually transmitted infection detection: C. trachomatis, HSV-2, M. genitalium, human RPP30-DNA detection
Application: Sexually transmitted infection detection
669.00€ *
Review
STI10 4-color (Bio-Rad) Multiplexed qPCR Assay
STI10 4-color (Bio-Rad) Multiplexed qPCR Assay

Item number: Cay40695-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme...
Keywords: Sexually transmitted infection detection: N. gonorrhoeae, T. pallidum, HSV-1, human RPP30-DNA detection
Application: Sexually transmitted infection detection
669.00€ *
Review
STI10 4-color (QuantStudio) Multiplexed qPCR Assay
STI10 4-color (QuantStudio) Multiplexed qPCR Assay

Item number: Cay40696-1

Oliogonucleotide recipes are optimized for QuantStudio 5,6,7,12K. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme...
Keywords: Sexually transmitted infection detection: N. gonorrhoeae, T. pallidum, HSV-1, human RPP30-DNA detection
Application: Sexually transmitted infection detection
669.00€ *
Review
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