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Positive Primer Set 1, Gene GAPDH
Item number: CT-800000
Chromatrap's Positive Primer Set 1 recognises the promoter region of the GAPDH gene that is associated with active transcription. A 69 base pair fragment is amplified with this primer set. It is a positive gene target for the following: H3K4me1, H3K4me3, H3K27ac and RNA Pol II, among others.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Positive Primer Set 2, Gene H19
Item number: CT-800001
Chromatrap's Positive Primer Set 2 amplifies a 134 base pair fragment of the imprinted H19 gene. It is a hypermethylated gene creating an appropriate positive target for methylation study. It is a positive gene target for 5-methylcytosine (5mC).
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Positive Primer Set 3, Gene MYT1
Item number: CT-800002
Chromatrap's Positive Primer Set 3 recognises a region proximal to the promoter of the zinc finger DNA-binding protein, Myt1, that is present when transcription is repressed. It is a positive gene target for the histone methylation mark H3K27me3 and EZH2.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Positive Primer Set 4, Gene SAT2
Item number: CT-800003
Chromatrap's Positive Primer Set 4 amplifies a 72 base pair fragment of the protein-coding SAT2 gene. This gene is present when transcription is repressed making it a positive gene target for the histone methylation mark H3K9me3.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Positive Primer Set 5, Gene FKBP5
Item number: CT-800004
Chromatrap's Positive Primer Set 5 recognises the promoter region of the protein-coding FkBP5 gene that has roles in glucocorticoid and progestin signalling pathways. It can regulate prostrate physiology in the presence of androgen (Magee et al. 2013). An 81 base pair fragment is amplified with this primer set. It is a positive gene target for the male hormone receptor gene...
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Positive Primer Set 6, Gene MLH1
Item number: CT-800005
Chromatrap's Positive Primer Set 6 recognises the promoter region of the DNA mismatch repair gene, MLH1. A 101 base pair product on human chromosome 3 is amplified by this primer set. It is a positive gene target for the transcription factor Sin3a.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Positive Primer Set 7, Gene Beta Globin
Item number: CT-800006
Chromatrap's Positive Primer Set 7 recognises the promoter region of the human B-Globin gene located on chromosome 11. A 121 base pair product is amplified with this primer set. It is a positive gene target for the histone acetylation mark H3K14ac and RNA Pol II.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Positive Primer Set 8, Gene IL8
Item number: CT-800007
Chromatrap's Positive Primer Set 8 amplifies a 78 base pair fragment of the Interleukin-8 gene, found on human chromosome 4. IL-8 is a pro-inflammatory chemokine, whose increased expression correlates with the progression of cancer. It is a positive gene target for the nuclear transcription factor NF-kB.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Negative Primer Set 1, Gene UBE2B
Item number: CT-800008
Chromatrap's Negative Primer Set 1 recognises the hypomethylated protein-coding gene UBE2B, this will not be picked up by methylation study assays. A 177 base pair fragment is amplified by this primer set. The enzyme that this gene encodes is required for DNA damage repair after replication has occurred. It is a negative gene target for 5-methylcytosine.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Negative Primer Set 2, Gene GAPDH
Item number: CT-800009
Chromatrap's Negative Primer Set 2 recognises the promoter of the GAPDH gene. A 69 base pair fragment is amplified with Negative Primer Set 2. It is a negative gene target for histone modifications that are associated with repressive transcription. This includes the following: H3K27me3, H3K9me3 and the transcription factor Sin3a.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Negative Primer Set 3, Gene Beta Globin
Item number: CT-800010
Chromatrap's Negative Primer Set 3 recognises the promoter of the human B-Globin gene. A 121 base pair fragment is amplified using this primer set. It is a negative gene target for active genes including H3K4me3 and RNA Poll II that are involved in initiating transcription, as well the Androgen Receptor (AR) gene.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
Negative Primer Set 4, SAT2
Item number: CT-800011
Chromatrap's Negative Primer Set 4 recognises the promoter region of the protein-coding SAT2 gene. A 72 base pair fragment is amplified by this primer set. It is a negative gene target for the acetylated histone mark H3K14ac, associated with active transcription.
| Application: | QPCR |
| Reactivity: | Human |
121.00€
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