Cells and Cell Lines

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial cultures of bacteria and yeast in sterile culture media allows selective growing of monoclonal cultures. From these cultures competent cells can be generated for the use in transformation. Cell culture colloquially refers to cultures of animal cells, in which we differentiate between ex vivo cultures and immortalized cell lines. Ex vivo cultures are derived from blood or organs and have a limited life span. Immortalized cell lines, however, are usually derived from tumors and proliferate indefinitely due to mutations in the cell cycle controls.

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial... read more »
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Cells and Cell Lines

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial cultures of bacteria and yeast in sterile culture media allows selective growing of monoclonal cultures. From these cultures competent cells can be generated for the use in transformation. Cell culture colloquially refers to cultures of animal cells, in which we differentiate between ex vivo cultures and immortalized cell lines. Ex vivo cultures are derived from blood or organs and have a limited life span. Immortalized cell lines, however, are usually derived from tumors and proliferate indefinitely due to mutations in the cell cycle controls.

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Normal Human Peripheral Blood Mononuclear Cells, Frozen
Normal Human Peripheral Blood Mononuclear Cells, Frozen

Item number: BPS-79059-1

Cryopreserved vial (30 x 10^6 cells or 100 x 10^6 cells, resp.) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated...
Keywords: PBMC, primary cells
Application: Immunotherapy studies
From 241.00€ *
Review
PD-1 - HEK293 Recombinant Cell Line
PD-1 - HEK293 Recombinant Cell Line

Item number: BPS-60680

Recombinant HEK293 stably expressing human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).
Keywords: CD279, hPD-1, Protein PD-1, Programmed cell death protein 1
Application: PD-1 binding moleculescreening, Cellular context, Antibody screening
Reactivity: Human
5,309.00€ *
Review
Anti-CD19 CAR / NFAT (Luciferase) Reporter Jurkat Cell Line (CD19 SCFV-CD28-4-1BB-CD3zeta)
Anti-CD19 CAR / NFAT (Luciferase) Reporter Jurkat Cell...

Item number: BPS-79853

Anti-CD19 CAR/NFAT-luciferase reporter Jurkat cell line is a double stable cell line expressing anti-CD19 CAR and NFAT-luciferase reporter. It is made from the anti-CD19 CAR lentivirus (BPS Bioscience #79851). The reporter cell line has been validated for anti CD19-CAR expression by FACS, and for luciferase reporter...
Application: CAR design validation
10,274.00€ *
Review
Anti-CD19 CAR negative control/ NFAT (Luciferase) Reporter Jurkat Cell Line (CD19 SCFV-CD28 transmem
Anti-CD19 CAR negative control/ NFAT (Luciferase)...

Item number: BPS-79854

Anti-CD19 CAR negative control/NFAT-luciferase reporter Jurkat cell line is a double stable cell line expressing anti-CD19 CAR negative control and NFAT-luciferase reporter. The anti-CD19 CAR negative control consists of anti-CD19 scFv linked to the CD28 transmembrane motif without any intracellular signaling...
Application: CAR design validation
2,041.00€ *
Review
Anti-CD19 CAR-CD4+ T cells (CD19 scFv-CD28-4-1BB-CD3zeta)
Anti-CD19 CAR-CD4+ T cells (CD19 scFv-CD28-4-1BB-CD3zeta)

Item number: BPS-79933

Anti-CD19 CAR-CD4+ T cells are ready-to-use CD4+ T cells infected with anti-CD19 CAR lentivirus (BPS Bioscience #79851). The anti-CD19 CAR consists of anti-CD19 scFv linked to the 3rd generation CAR (Chimeric Antigen Receptor) containing CD28, 4-1BB, and CD3zeta domains. CD4+ T cells (BPS Bioscience #79752) were...
Keywords: Cluster of Differentiation 19, Surface Antigen B4, CVID3
Application: Control, Proof of concept studies
2,453.00€ *
Review
Anti-CD19 CAR-CD8+ T cells (CD19 scFv-CD28-4-1BB-CD3zeta)
Anti-CD19 CAR-CD8+ T cells (CD19 scFv-CD28-4-1BB-CD3zeta)

Item number: BPS-79934

Anti-CD19 CAR-CD8+ T cells are ready-to-use CD8+ T cells infected with anti-CD19 CAR lentivirus (BPS Bioscience #79851). The anti-CD19 CAR consists of anti-CD19 scFv linked to the 3rd generation CAR (Chimeric Antigen Receptor) containing CD28, 4-1BB, and CD3zeta domains. CD8+ T cells (BPS Bioscience #79753) were...
Keywords: Cluster of Differentiation 19, Surface Antigen B4, CVID3
Application: Control, Proof of concept studies
2,453.00€ *
Review
Nrf2 Leeporter(TM) MCF7 Cell Line
Nrf2 Leeporter(TM) MCF7 Cell Line

Item number: ABE-14-117ACL-NP

The price shown is for non-profit organizations (NP). Profit-oriented organizations please ask for the price. The Nrf2 Leeporter cell line is a stably transfected MCF7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the antioxidant response element (ARE). ARE is known...
Application: FA
From 2,090.00€ *
Review
BirA-transformed Competent E. coli cells
BirA-transformed Competent E. coli cells

Item number: BPS-27462

BirA-transformed Competent E. coli cells are supplied as 10 x 100 µl vials. Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene. These cells are compatible with most cloning vectors and are...
Keywords: bioR, b3973, Biotin--protein ligase, Biotin operon repressor, Bifunctional ligase/repressor BirA,...
Application: Biotin labelling of recombinant proteins
537.00€ *
Review
RARalpha Reporter Cell Line (2x10(6) cells)
RARalpha Reporter Cell Line (2x10(6) cells)

Item number: BPS-60503

2x10(6) cells. The RARalpha Reporter (Luc)-HEK293 Cell Line is designed for monitoring the activity of retinoic acid receptor alpha (RARalpha). The RARalpha Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells...
Application: RARalpha reporter assays
Reactivity: Human
2,041.00€ *
Review
NKp46 Stable Cell Line
NKp46 Stable Cell Line

Item number: ABE-14-512ACL-NP

The price shown is for non-profit organizations (NP). Profit-oriented organizations please ask for the price. NKp46 Stable Cell Line
Application: FA
From 2,280.00€ *
Review
GITR - HEK293 Recombinant Cell Line
GITR - HEK293 Recombinant Cell Line

Item number: BPS-79092

This cell line expresses human GITR (glucocorticoid-induced TNFR family-related gene, TNFRSF18, CD357), Genbank Accession Number NM_ 004195.2.
Application: Activator / inhibitor screening
Reactivity: Human
5,200.00€ *
Review
Gli Reporter - NIH3T3 Cell line (Hedgehog Pathway)
Gli Reporter - NIH3T3 Cell line (Hedgehog Pathway)

Item number: BPS-60409

The Gli Reporter - NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. The hedgehog pathway controls stem cell growth in embryonic and adult tissues and promotes tumor growth in a number of human cancers. The mammalian hedgehog proteins, Sonic Hedgehog (Shh), Indian Hedgehog...
Application: Hedgehog pathway activity monitoring
2,221.00€ *
Review
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