Cells and Cell Lines

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial cultures of bacteria and yeast in sterile culture media allows selective growing of monoclonal cultures. From these cultures competent cells can be generated for the use in transformation. Cell culture colloquially refers to cultures of animal cells, in which we differentiate between ex vivo cultures and immortalized cell lines. Ex vivo cultures are derived from blood or organs and have a limited life span. Immortalized cell lines, however, are usually derived from tumors and proliferate indefinitely due to mutations in the cell cycle controls.

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial... read more »
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Cells and Cell Lines

Cell culture and microbial culture techniques are essential for almost every aspect of biological research since modern microbiology started in the 19th century. The invention of microbial cultures of bacteria and yeast in sterile culture media allows selective growing of monoclonal cultures. From these cultures competent cells can be generated for the use in transformation. Cell culture colloquially refers to cultures of animal cells, in which we differentiate between ex vivo cultures and immortalized cell lines. Ex vivo cultures are derived from blood or organs and have a limited life span. Immortalized cell lines, however, are usually derived from tumors and proliferate indefinitely due to mutations in the cell cycle controls.

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HBZY-1 cell line
HBZY-1 cell line

Item number: E-EP-CL-0092.1

Species: Rattus norvegicus, Rat. Tissue: Kidney. Morphology: Epithelial. Growth properties: Adherent. Biosafety: 1. Medium: DMEM+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to...
Reactivity: Rat
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Hs-746T cell line
Hs-746T cell line

Item number: E-EP-CL-0115.1

Species: Homo sapiens, human. Age: Male,74 years. Tissue: Stomach, derived from metastatic site: left leg. Morphology: Epithelial. Growth properties: Adherent. Biosafety: 1. Medium: DMEM+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all...
Reactivity: Human
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MRC-5 Cell Line
MRC-5 Cell Line

Item number: E-EP-CL-0161.1

Background: The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus in 1966. The cells are capable of 42 to 46 population doublings before the onset of senescence. This is a normal diploid human cell line with 46, XY karyotype. The modal chromosome number was 46, occurring in 70% of...
Reactivity: Human
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P388D1 cell line
P388D1 cell line

Item number: E-EP-CL-0180.1

Species: Mus musculus, mouse. Age: Female. Tissue: Lymphoma. Morphology: Lymphoblast. Growth properties: Suspension. Biosafety: 1. Medium: DMEM+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin...
Reactivity: Mouse
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SCaBER cell line
SCaBER cell line

Item number: E-EP-CL-0203.1

Species: Homo sapiens, human. Age: Male,58 years. Tissue: Urinary bladder. Morphology: Epithelial. Growth properties: Adherent. Biosafety: 1. Medium: MEM+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains...
Reactivity: Human
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Hela S3 cell line
Hela S3 cell line

Item number: E-EP-CL-0350.1

Background: HeLa S3 is a clonal derivative of the parent HeLa line. S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura. Species: Homo sapiens, human. Age: Female,31 years. Tissue: Cervix. Morphology: Epithelial. Growth properties: Adherent. Doubling time: ~48 hours. Biosafety: 2. Medium: Ham's...
Reactivity: Human
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NCI-H1688 cell line
NCI-H1688 cell line

Item number: E-EP-CL-0389.1

Species: Homo sapiens, Human. Age: 50 years. Tissue: Lung, derived from metastatic site: Liver. Morphology: Epithelial. Growth properties: Adherent. Biosafety: 1. Medium: RPMI-1640+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of...
Reactivity: Human
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NCI-H1703 cell line
NCI-H1703 cell line

Item number: E-EP-CL-0390.1

Background: This cell line was isolated from the same patient as was NCI-H1693. Species: Homo sapiens, Human. Age: 54 years adult. Tissue: Lung. Morphology: Epithelial. Growth properties: Adherent. Biosafety: 1. Medium: RPMI-1640+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell...
Reactivity: Human
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NCI-H716 [H716] cell line
NCI-H716 [H716] cell line

Item number: E-EP-CL-0405.1

Species: Homo sapiens, human. Age: Male,33 years. Tissue: Cecum. Morphology: Epithelial. Growth properties: Suspension, multicell aggregates and some adherent cells. Doubling time: ~50 hours. Biosafety: 1. Medium: RPMI-1640+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer...
Reactivity: Human
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RAG cell line
RAG cell line

Item number: E-EP-CL-0421.1

Species: Mus musculus, mouse. Tissue: Kidney. Morphology: Amoeboid. Growth properties: Adherent. Biosafety: 1. Medium: MEM+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL...
Reactivity: Mouse
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RKO-AS45-1 cell line
RKO-AS45-1 cell line

Item number: E-EP-CL-0425.1

Background: The RKO-AS45-1 cell line was generated from the colon carcinoma RKO cell line by transfection with the open reading frame portions of human cDNAs for GADD45 cloned into the expression vector pCMV. Species: Homo sapiens, human. Tissue: Colon. Morphology: Epithelial. Growth properties: Adherent. Biosafety:...
Reactivity: Human
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MA-104 [MA 104, MA104] cell line
MA-104 [MA 104, MA104] cell line

Item number: E-EP-CL-0479.1

Background: This cell line was established from an African Green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11. Species: Cercopithecus aethiops. Age: Fetus. Tissue: Kidney. Morphology: Epithelial. Growth properties: Adherent. Biosafety: 1. Medium: DMEM+10% FBS+1% P/S. Subculturing:...
Reactivity: Grivet
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