Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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Anti-Green Fluorescent Protein (GFP)
Anti-Green Fluorescent Protein (GFP)

Item number: 600-101-215

GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against...
Keywords: Anti-GFP, Anti-Green fluorescent protein
Application: ELISA, IHC, IFM, IP, WB
Host: Goat
Species reactivity: broad
From 180.00€ *
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Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone eB182
Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone...

Item number: 18-8816-31

Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate...
Keywords: TrueBlot®, IP/WB
Application: WB
Host: Mouse
Species reactivity: rabbit
186.00€ * 139.50€ *
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Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone eB182
Anti-Rabbit IgG HRP conjugated (Rabbit TrueBlot®), clone...

Item number: 18-8816-33

Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate...
Keywords: TrueBlot®, IP/WB
Application: WB
Host: Mouse
Species reactivity: rabbit
374.00€ * 280.50€ *
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Anti-Mouse IgG HRP conjugated (Mouse TrueBlot® ULTRA), clone eB144
Anti-Mouse IgG HRP conjugated (Mouse TrueBlot® ULTRA),...

Item number: 18-8817-33

Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality...
Keywords: TrueBlot®, IP/WB
Application: IFM, IP, WB
Host: Rat
Species reactivity: mouse
374.00€ * 280.50€ *
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Anti-Rabbit IgG (H&L) [Goat] Peroxidase conjugated
Anti-Rabbit IgG (H&L) [Goat] Peroxidase conjugated

Item number: 611-1302

Anti-Rabbit IgG Antibody peroxidase generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: rabbit
204.00€ *
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Anti-Mouse IgG HRP conjugated (Mouse TrueBlot® ULTRA), clone eB144
Anti-Mouse IgG HRP conjugated (Mouse TrueBlot® ULTRA),...

Item number: 18-8817-31

Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality...
Keywords: TrueBlot®, IP/WB
Application: IFM, IP, WB
Host: Rat
Species reactivity: mouse
186.00€ * 139.50€ *
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Western Blot of TrueBlot® Anti-Rabbit Ig IP Agarose Beads. Lane 1: Protein Standard Opal Pre-stained (p/n MB-210-0500). Lane 2: Rb-a-GFP Input. Lane 3: Rb-a-GFP Unbound. Lane 4: Rb-a-Elute. Primary Antibody: TrueBlot® Anti-Rabbit Ig IP Agarose Beads (00-8
Anti-Rabbit Ig IP Beads (TrueBlot®)

Item number: 00-8800-25

TrueBlot® anti-Rabbit Ig IP Beads are a suspension of activated agarose beads coupled with goat anti-rabbit IgG. It is suitable for precipitation of rabbit IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently...
Keywords: TrueBlot®, IP/WB
Application: IP, WB
Host: Goat
Species reactivity: rabbit
317.00€ * 237.75€ *
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Anti-Rabbit IgG (H&L) [Goat]
Anti-Rabbit IgG (H&L) [Goat]

Item number: 611-1102

Format - Whole IgG
Application: ELISA, IHC, IFM, IP, WB
Host: Goat
Species reactivity: rabbit
160.00€ *
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Anti-Mouse IgG (H&L) [DONKEY], DyLight 549 conjugated
Anti-Mouse IgG (H&L) [DONKEY], DyLight 549 conjugated

Item number: 610-742-002

DyLight(TM) dyes are characterized by high fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Donkey
Species reactivity: mouse
160.00€ *
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Anti-Mouse IgG HRP conjugated (Mouse TrueBlot® ULTRA), clone eB144
Anti-Mouse IgG HRP conjugated (Mouse TrueBlot® ULTRA),...

Item number: 18-8817-30

Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. Mouse IgG TrueBlot® ULTRA is provided as 1000X...
Application: IFM, IP, WB
Host: Rat
Species reactivity: mouse
95.00€ * 71.25€ *
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Anti-Human IgM (mu chain), DyLight 800 conjugated
Anti-Human IgM (mu chain), DyLight 800 conjugated

Item number: 609-145-007

DyLight(TM) dyes are characterized by high fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: human
160.00€ *
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Anti-Mouse IgG (H&L) [Goat] Peroxidase-Conjugate
Anti-Mouse IgG (H&L) [Goat] Peroxidase-Conjugate

Item number: 610-1302

Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: mouse
235.00€ *
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