Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the... read more »
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Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

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TBARS Assay Kit
TBARS Assay Kit

Item number: Cay10009055-96

Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from PUFAs results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with...
Keywords: Thiobarbituric Acid Reactive Substances
Application: Lipid peroxidation
291.00€ *
Review
Glucose Colorimetric Assay Kit
Glucose Colorimetric Assay Kit

Item number: Cay10009582-192

Glucose, a monosaccharide (or simple sugar), is the most important carbohydrate in biology. Transported via the blood stream, it is the primary source of energy for the body's cells. Glucose level is tightly regulated in the human body. Failure to maintain blood glucose in the normal range leads to conditions of...
Application: Glucose detection
274.00€ *
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Triglyceride Colorimetric Assay Kit
Triglyceride Colorimetric Assay Kit

Item number: Cay10010303-96

The measurement of triglyceride levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman's Triglyceride Assay Kit provides a simple, reproducible, and sensitive tool for assaying triglycerides in plasma,...
Keywords: TG
Application: Triglyceride detection
308.00€ *
Review
Creatinine (urinary) Colorimetric Assay Kit
Creatinine (urinary) Colorimetric Assay Kit

Item number: Cay500701-480

Cayman's Creatinine (urinary) Colorimetric Assay Kit can be used to measure creatinine levels in urine. The assay relies on the Jaffe reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate. The color derived from creatinine is then destroyed at acidic pH. The difference in...
Application: Creatinine measurement
From 129.00€ *
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COX (ovine/human) Inhibitor Screening Assay Kit
COX (ovine/human) Inhibitor Screening Assay Kit

Item number: Cay560131-96

The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2alpha by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via enzyme immunosorbent assay (ELISA) using an antiserum specific to PGF2alpha. This assay includes both ovine COX-1 and human...
Application: COX inhibitor screening
584.00€ *
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NETosis Assay Kit
NETosis Assay Kit

Item number: Cay601010-1

Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of NETs, as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either...
Keywords: NET, Citrullination
Application: NET generation assay
484.00€ *
Review
beta-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
beta-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

Item number: Cay700190-96

beta-Hydroxybutyrate (beta-HB, 3-hydroxybutyric acid) is a "ketone body" which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. The term 'ketone body' refers to three molecules, acetoacetate, beta-HB, and acetone. beta-HB and...
Keywords: 3-Hydroxybutyric Acid, beta-HB
Application: beta-HB measurement
352.00€ *
Review
TBARS (TCA Method) Assay Kit
TBARS (TCA Method) Assay Kit

Item number: Cay700870-96

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable and decompose to form a complex series of compounds, which include reactive carbonyl compounds, such...
Keywords: Thiobarbituric Acid Reactive Substances (TCA Method)
Application: Lipid peroxidation assay
234.00€ *
Review
Glutathione Assay Kit
Glutathione Assay Kit

Item number: Cay703002-96

Cayman's GSH assay kit utilizes a carefully optimized enzymatic recycling method, using glutathione reductase for the quantification of GSH. The sulfhydryl group of GSH reacts with DTNB (5,5'-dithio-bis-2-nitrobenzoic acid, Ellman's reagent) and produces a yellow colored 5-thio-2-nitrobenzoic acid (TNB). The mixed...
Keywords: GSH Assay Kit
Application: GSH quantification
From 360.00€ *
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Glutathione Peroxidase Assay Kit
Glutathione Peroxidase Assay Kit

Item number: Cay703102-96

Glutathione peroxidase (GPX) catalyzes the reduction of hydroperoxides, including hydrogen peroxides, by reduced glutathione and functions to protect the cell from oxidative damage. With the exception of phospholipid-hydroperoxide GPX, a monomer, all of the GPX enzymes are tetramers of four identical subunits. Each...
Keywords: GPX Assay Kit
Application: GTx activity detection
From 444.00€ *
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Superoxide Dismutase Assay Kit
Superoxide Dismutase Assay Kit

Item number: Cay706002-96

Significant amounts of superoxide dismutase (SOD) in cellular and extracellular environments are crucial for the prevention of diseases linked to oxidative stress. Mutations in SOD account for approximately 20% of familial amyotrophic lateral sclerosis (ALS) cases. SOD also appears to be important in the prevention...
Keywords: SOD Assay Kit
Application: SOD detection
From 352.00€ *
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Catalase Assay Kit
Catalase Assay Kit

Item number: Cay707002-96

Catalase (EC 1.11.1.6, 2H2O2 oxidoreductase) is an ubiquitous antioxidant enzyme that is present in most aerobic cells. Catalase (CAT) is involved in the detoxification of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), which is a toxic product of both normal aerobic metabolism and pathogenic ROS...
Keywords: CAT
Application: Catalase activity determination
From 422.00€ *
Review
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