Cloning and Expression Kits

The BPS Bioscience PCR cloning kit allows for the insertion of multiple DNA fragments into the desired vector in a single reaction, no ligation step is required, saving time and effort. Simply mix the purified PCR product with the vector in a 1:1 molar ratio (Total DNA : Vector) and follow the protocol. Efficiencies of >90% are routinely obtained. Available with or without...

The BPS Bioscience PCR cloning kit allows for the insertion of multiple DNA fragments into the desired vector in a single reaction, no ligation step is required, saving time and effort. Simply mix the purified PCR product with the vector in a 1:1 molar ratio (Total DNA : Vector) and follow the protocol. Efficiencies of >90% are routinely obtained. Available with or without...

The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L1 (GenBank Accession #NM_014143) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells (BPS #60535) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and screen for modulators...

The CRE/CREB Reporter kit is designed for monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready CRE luciferase reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response element (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level...

The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L2 (GenBank Accession #NM_025239) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells (BPS #60535) to study the interactions of PD-1 with PD-L2 ligand in a cellular context and screen for modulators...

Assay Genie's Classic CRISPR sgRNA Synthesis Kit (S. pyogenes) is the perfect tool for generating the highest quality sgRNAs for the spCas9 system. * Product SKU* BN00129* Size* 25 Reactions* Kit Summary* For generating the highest quality sgRNAs for the spCas9 system. * Applications* sgRNA in vitro studies * sgRNA in vitro screening * Features & Benefits* Easy streamlined...

Isolation of high quality DNA from mammalian cells and tissue in sufficient quantities is important for many basic research and clinical applications. Assay Genie's Mammalian Cell Genomic DNA Isolation Kit facilitates purification of quality DNA from mammalian cultured cells or tissue. This kit utilizes enzymatic reactions to release genomic DNA from the cells. Upon release,...

Assay Genie's CRISPR Genomic Cleavage Detection Kit is designed as an easy and yet effective way to verify your genomic editing process. CRISPR-edited cell samples are used as a template in PCR reactions targeting the specific region of interest. * Product SKU* BN00127* Size* 25 Reactions* Kit Summary* To verify your genomic editing process* Applications* Genome Editing *...

Assay Genie's Express CRISPR sgRNA Synthesis Kit (S. pyogenes) is a neat handy tool for generating sgRNAs for spCas9 system.* Product SKU* BN00130* Size* 25 Reactions* Kit Summary* A neat handy tool for generating sgRNAs for spCas9 system. * Applications* sgRNA in vitro studies * sgRNA in vitro screening * Features & Benefits* Easy 1-step setup* Short reaction time, only 30...

Assay Genie's Classic CRISPR sgRNA Synthesis Kit (S. pyogenes) is the perfect tool for generating the highest quality sgRNAs for the spCas9 system. * Product SKU* BN00128* Size* 25 Reactions* Kit Summary* For generating the highest quality sgRNAs for the spCas9 system. * Applications* sgRNA in vitro studies * sgRNA in vitro screening * Features & Benefits* Easy streamlined...