Sample Preparation Kits

When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman's MitoCheck(R) Mitochondrial (Tissue) Isolation Kit includes a complete package of...

Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and...

Many lipids have a free carboxylic acid group that typically requires a weak acid mobile phase to ensure retention by HPLC. Unfortunately, the presence of the acid in the mobile phase also suppresses anion formation and often leads to poor electrospray ionization and poor MS sensitivity. Cayman's AMP+ Mass Spectrometry Kit contains a positively charged reagent (AMP+) that...

The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates. Therefore, deproteinization is a necessary step in many procedures prior to analysis of biological samples. AAT Bioquest's Deproteinization Sample Preparation Kit offers a rapid...

Preparation of nuclear extracts is the first step in examining transcription factor activity. Cayman's Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in Cayman's Transcription Factor Assay kits. The proteins isolated using this kit can also...

Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. This kit is designed to maximize extraction of small fragments of DNA with minimal contamination from intact chromatin. The isolated DNA is separated by electrophoresis and can be visualized using ethidium bromide.

These N-terminal tagged proteins are potent, irreversible and specific inhibitors of most deconjugating enzymes including ubiquitin C-terminal hydrolases (UCHs), ubiquitin specific proteases (USPs), Apg4b, SENPs and NEDPs. They are useful for inhibiting the hydrolysis of Ubiquitin/UBL chains or Ubiquitin/UBL monomers on substrate proteins in vitro. The HA peptide sequence...

HSP60 (also known as Chaperonin 60) and HSP10 (also known as Chaperonin 10) are the eukaryotic homologues of prokaryotic GroEL and GroES chaperones, respectively. These proteins are found mainly in mitochondria, but can also be detected in cytosol and extracellular fluids including peripheral blood. The HSP60/HSP10 complex plays an essential role in the translocation and...

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSP70 function depends on its cycling between two states: ATP-bound...

This kit contains all reagents necessary for simple one-step purification of 8-isoprostane from most biological samples.