Sample Preparation Kits

Many lipids have a free carboxylic acid group that typically requires a weak acid mobile phase to ensure retention by HPLC. Unfortunately, the presence of the acid in the mobile phase also suppresses anion formation and often leads to poor electrospray ionization and poor MS sensitivity. Cayman's AMP+ MaxSpec(TM)...

Preparation of nuclear extracts is the first step in examining transcription factor activity. Cayman's Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in Cayman's Transcription Factor...

When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman's MitoCheck(R) Mitochondrial...

Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme,...

The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates. Therefore, deproteinization is a necessary step in many procedures prior to analysis of biological samples. AAT Bioquest's...

The presence of protein and various enzyme activities frequently interferes with the analysis of small molecules in biological samples. Many bioassays require removal of proteins from samples prior to analysis. Among the deproteinization protocols developed over the last half century, perchloric acid (PCA)...

The study of biofluids, cell, bacteria, yeast, and tissue lysates has delivered vital information regarding their biochemical composition. These biological samples comprise a rich, complex list of lipids, proteins, small organic molecules, ions, and, depending on the sample, exogenous molecules (i.e. drugs). Several...

This kit contains all reagents necessary for simple one-step purification of 8-isoprostane from most biological samples.

C18 columns are used for extraction of peptidic biomarker of interest from their matrix., They are for single-use only, they can not be regenerated.

Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. This kit is designed to maximize extraction of small fragments of DNA with minimal contamination from intact chromatin. The isolated DNA is separated by electrophoresis and can be...

Description:, BioAssay(TM) Total Protein Extraction Kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications including SDS-PAGE, Western Blot, gel mobility shift, protein...

The angiotensin II (AII) Inhibition Cocktail has been developped by Bertin Bioreagent in order to fix physiological concentration of Angiotensin II in biological samples namely blood. Indeed, it has been observed that sample preparation is highly recommanded when dosing Angiotensin II for preventing the formation or...