qPCR Gene Primers

qPCR gene primers are validated primer sets for quantitative real-time PCR. The primers are suitable for expression analysis and optimized for the SYBR® green method. The amplified fragments are in the range of 100-300 base pairs. The primer sequences are shipped with the products.

qPCR gene primers are validated primer sets for quantitative real-time PCR. The primers are suitable for expression analysis and optimized for the SYBR® green method. The amplified fragments are... read more »
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qPCR Gene Primers

qPCR gene primers are validated primer sets for quantitative real-time PCR. The primers are suitable for expression analysis and optimized for the SYBR® green method. The amplified fragments are in the range of 100-300 base pairs. The primer sequences are shipped with the products.

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Positive Primer Set 1, Gene GAPDH
Positive Primer Set 1, Gene GAPDH

Item number: CT-800000

Chromatrap's Positive Primer Set 1 recognises the promoter region of the GAPDH gene that is associated with active transcription. A 69 base pair fragment is amplified with this primer set. It is a positive gene target for the following: H3K4me1, H3K4me3, H3K27ac and RNA Pol II, among others.
Application: QPCR
Reactivity: Human
121.00€
Review
Positive Primer Set 2, Gene H19
Positive Primer Set 2, Gene H19

Item number: CT-800001

Chromatrap's Positive Primer Set 2 amplifies a 134 base pair fragment of the imprinted H19 gene. It is a hypermethylated gene creating an appropriate positive target for methylation study. It is a positive gene target for 5-methylcytosine (5mC).
Application: QPCR
Reactivity: Human
121.00€
Review
Positive Primer Set 3, Gene MYT1
Positive Primer Set 3, Gene MYT1

Item number: CT-800002

Chromatrap's Positive Primer Set 3 recognises a region proximal to the promoter of the zinc finger DNA-binding protein, Myt1, that is present when transcription is repressed. It is a positive gene target for the histone methylation mark H3K27me3 and EZH2.
Application: QPCR
Reactivity: Human
121.00€
Review
Positive Primer Set 4, Gene SAT2
Positive Primer Set 4, Gene SAT2

Item number: CT-800003

Chromatrap's Positive Primer Set 4 amplifies a 72 base pair fragment of the protein-coding SAT2 gene. This gene is present when transcription is repressed making it a positive gene target for the histone methylation mark H3K9me3.
Application: QPCR
Reactivity: Human
121.00€
Review
Positive Primer Set 5, Gene FKBP5
Positive Primer Set 5, Gene FKBP5

Item number: CT-800004

Chromatrap's Positive Primer Set 5 recognises the promoter region of the protein-coding FkBP5 gene that has roles in glucocorticoid and progestin signalling pathways. It can regulate prostrate physiology in the presence of androgen (Magee et al. 2013). An 81 base pair fragment is amplified with this primer set. It is a positive gene target for the male hormone receptor gene...
Application: QPCR
Reactivity: Human
121.00€
Review
Positive Primer Set 6, Gene MLH1
Positive Primer Set 6, Gene MLH1

Item number: CT-800005

Chromatrap's Positive Primer Set 6 recognises the promoter region of the DNA mismatch repair gene, MLH1. A 101 base pair product on human chromosome 3 is amplified by this primer set. It is a positive gene target for the transcription factor Sin3a.
Application: QPCR
Reactivity: Human
121.00€
Review
Positive Primer Set 7, Gene Beta Globin
Positive Primer Set 7, Gene Beta Globin

Item number: CT-800006

Chromatrap's Positive Primer Set 7 recognises the promoter region of the human B-Globin gene located on chromosome 11. A 121 base pair product is amplified with this primer set. It is a positive gene target for the histone acetylation mark H3K14ac and RNA Pol II.
Application: QPCR
Reactivity: Human
121.00€
Review
Positive Primer Set 8, Gene IL8
Positive Primer Set 8, Gene IL8

Item number: CT-800007

Chromatrap's Positive Primer Set 8 amplifies a 78 base pair fragment of the Interleukin-8 gene, found on human chromosome 4. IL-8 is a pro-inflammatory chemokine, whose increased expression correlates with the progression of cancer. It is a positive gene target for the nuclear transcription factor NF-kB.
Application: QPCR
Reactivity: Human
121.00€
Review
Negative Primer Set 1, Gene UBE2B
Negative Primer Set 1, Gene UBE2B

Item number: CT-800008

Chromatrap's Negative Primer Set 1 recognises the hypomethylated protein-coding gene UBE2B, this will not be picked up by methylation study assays. A 177 base pair fragment is amplified by this primer set. The enzyme that this gene encodes is required for DNA damage repair after replication has occurred. It is a negative gene target for 5-methylcytosine.
Application: QPCR
Reactivity: Human
121.00€
Review
Negative Primer Set 2, Gene GAPDH
Negative Primer Set 2, Gene GAPDH

Item number: CT-800009

Chromatrap's Negative Primer Set 2 recognises the promoter of the GAPDH gene. A 69 base pair fragment is amplified with Negative Primer Set 2. It is a negative gene target for histone modifications that are associated with repressive transcription. This includes the following: H3K27me3, H3K9me3 and the transcription factor Sin3a.
Application: QPCR
Reactivity: Human
121.00€
Review
Negative Primer Set 3, Gene Beta Globin
Negative Primer Set 3, Gene Beta Globin

Item number: CT-800010

Chromatrap's Negative Primer Set 3 recognises the promoter of the human B-Globin gene. A 121 base pair fragment is amplified using this primer set. It is a negative gene target for active genes including H3K4me3 and RNA Poll II that are involved in initiating transcription, as well the Androgen Receptor (AR) gene.
Application: QPCR
Reactivity: Human
121.00€
Review
Negative Primer Set 4, SAT2
Negative Primer Set 4, SAT2

Item number: CT-800011

Chromatrap's Negative Primer Set 4 recognises the promoter region of the protein-coding SAT2 gene. A 72 base pair fragment is amplified by this primer set. It is a negative gene target for the acetylated histone mark H3K14ac, associated with active transcription.
Application: QPCR
Reactivity: Human
121.00€
Review
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