Buffer Recipes
Western Blotting
10x Transfer Buffer
- Tris, ultra pure: 15.2 g
- Glycine, ultra pure: 72.1 g
- SDS, ultra pure: 5.0 g
- Ultra pure water to 500 ml
Store at 4°C.
1x Transfer Buffer
- 10x Transfer Buffer: 50 ml
- Methanol: 100 ml
- Distilled water: 350 ml
Make fresh for each use.
TBST
- Tris, ultra pure: 6.1 g
- NaCl: 8.68 g
- Tween 20: 500 µl
Adjust the volume to 1 liter with distilled water. Adjust pH to 8.0 with HCL. Store at 4 - 25°C.
SDS PAGE
20x Running Buffer
- Tricine, ultra pure: 71.7 g
- Tris, ultra pure: 72.6 g
- SDS, ultra pure: 10.0 g
- Sodium bisulfite: 2.5 g
- Ultra pure water to 500 ml
Store at 4°C. For 1x Running Buffer, add 10 ml of 20x Running Buffer to 190 ml of distilled water.
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