Buffer Recipes

Western Blotting

10x Transfer Buffer

Store at 4°C.

1x Transfer Buffer

  • 10x Transfer Buffer: 50 ml
  • Methanol: 100 ml
  • Distilled water: 350 ml

Make fresh for each use.

Blocking Buffer

Make fresh for each use.

TBST

Adjust the volume to 1 liter with distilled water. Adjust pH to 8.0 with HCL. Store at 4 - 25°C.

Stripping Buffer

Adjust the volume to 1 liter with distilled water. Adjust pH to 2.2.

SDS PAGE

20x Running Buffer

Store at 4°C. For 1x Running Buffer, add 10 ml of 20x Running Buffer to 190 ml of distilled water.

6X Laemmli (Sample) Buffer

Adjust the volume to 10 mL with distilled water. Store at -20 °C in 0.5 mL aliquots.

Coomassie Brilliant Blue Solution

20X MOPS SDS Running Buffer

Adjust the volume to 1 liter with distilled water

20X MES SDS Running Buffer

Adjust the volume to 1 liter with distilled water.

General Use

10x PBS

  • NaCl: 8 g
  • KCl: 0.2 g
  • Disodium phosphate: 1.44 g
  • Monopotassium phosphate: 245 mg

Adjust the volume to 1 liter with distilled water. Adjust pH to 7.4.

Agarose Gel Electrophoresis

10X DNA loading buffer

Adjust the volume to 10 mL with distilled water.

50X TAE

Adjust the volume to 1 liter with distilled water.

10X TBE

Adjust the volume to 1 liter with distilled water.

Southern and Northern Blot

20X SSC Buffer

  • NaCl: 175.3 g
  • Sodium citrate: 88.2 g

Adjust the volume to 1 liter with distilled water. Adjust pH to 7.0 with HCl.

20X SSPE Buffer

  • Sodium citrate: 175.3 g
  • Monosodium phosphate: 27.6 g
  • EDTA: 7.4 g

Adjust the volume to 1 liter with distilled water. Adjust pH to 7.4 with NaOH.

Sedimentation

Sucrose Gradient Buffer

  • HEPES, ultra pure: 0.691 g
  • Potassium acetate: 1.47 g
  • Magnesium acetate: 0.214 g
  • β-Mercaptoethanol: 800 µL

Adjust the volume to 100 mL with distilled water.

Sucrose Stock Solution

Adjust the volume to 100 mL with distilled water.

Immunoprecipitation

RIPA buffer

Adjust the volume to 100 mL with distilled water. Add 1 mM PMSF before each use