Conjugation Kits

Ex: 403 nm. Em: 454 nm. Soluble in: DMSO. AAT Bioquest's mFluorT dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluorT Violet 450 dyes have fluorescence excitation and...

Ex (nm) 491, Em (nm) 514. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor(TM) 488 dyes...

Lipophilic fluorescent stain. DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells.

Ex: 494 nm. Em: 517 nm. Soluble in: DMSO. The fluorescent dye azides react with alkynes via a copper-catalyzed click reaction (CuAAC). This click chemistry is increasingly being used in a variety of biological applications. AF488 alkyne has the same chemical structure to Alexa Fluor® 488 alkyne (Alexa Fluor® is the...

Ex: 651 nm. Em: 700 nm. Soluble in: DMSO. APC-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~700 nm. AAT Bioquest offers this BuccutiteT rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as...

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor(TM) 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with...

Ex: 555 nm. Em: 575 nm. The Cell Navigator(TM) Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. It may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for...

Ex: 358 nm. Em: 450 nm. Soluble in: DMSO. DiFMUP is a fluorinated MUP, which is a commonly used fluorogenic alkaline phosphatase substrate. The reaction product of DiFMUP has excitation/emission maxima of ~358/450 nm, essentially identical to the spectral properties of MUP. Upon phosphatase-induced hydrolysis DiFMUP...

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. iFluor(TM) 700 dyes have fluorescence excitation and emission maxima close to 690 nm and 710 nm respectively. These...

Ex: 543 nm. Em: 571 nm. Soluble in: DMSO. 5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more...

Ex: 416 nm. Em: 451 nm. Soluble in: DMSO. PacBlue succinimidyl ester is the same molecule to Pacific BlueT succinimidyl ester (Pacific BlueT is the trademark of ThermoFisher). The amine-reactive Pac Blue succinimidyl ester can be used to prepare the blue fluorescent antibody conjugates that can be well excited by...

Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production. Mariculture keyhole limpet hemocyanin (mcKLH) is a hemocyanin from the Concholepas concholepas mollusk with immunogenic properties similar to KLH but is more stable and efficient as a...