Conjugation Kits

Ex: 403 nm. Em: 454 nm. Soluble in: DMSO. AAT Bioquest's mFluorT dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluorT Violet 450 dyes have fluorescence excitation and emission maxima of ~405 nm and ~450 nm respectively. These...

Ex (nm) 491, Em (nm) 514. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor(TM) 488 dyes (Ex/Em=497 nm/525 nm) are far superior compared to conjugates...

Lipophilic fluorescent stain. DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells.

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor(TM) 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with good photostability. Our in-house comparable studies indicated...

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. iFluor(TM) 700 dyes have fluorescence excitation and emission maxima close to 690 nm and 710 nm respectively. These spectral characteristics make them an excellent alternative to...

Ex: 543 nm. Em: 571 nm. Soluble in: DMSO. 5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more stable. Cautions must be exercised for the storage of TRITC...

Ex: 416 nm. Em: 451 nm. Soluble in: DMSO. PacBlue succinimidyl ester is the same molecule to Pacific BlueT succinimidyl ester (Pacific BlueT is the trademark of ThermoFisher). The amine-reactive Pac Blue succinimidyl ester can be used to prepare the blue fluorescent antibody conjugates that can be well excited by the 405 nm spectral line of the blue diode (violet) laser....

Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production. Mariculture keyhole limpet hemocyanin (mcKLH) is a hemocyanin from the Concholepas concholepas mollusk with immunogenic properties similar to KLH but is more stable and efficient as a carrier protein for the production of antibodies to haptens and...

AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 555 dyes have fluorescence excitation and emission maxima of 550 nm and 570 nm...

Ex: 651 nm. Em: 662 nm. Soluble in: DMSO. APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm. AAT Bioquest offers this BuccutiteT rapid labeling kit to facilitate the APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. BuccutiteT APC Conjugation Kit provides a...

AAT Bioquest's iFluor(TM) dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. iFluor(TM) 790 dyes have fluorescence excitation and emission maxima close to 790 nm and 810 nm respectively. These spectral characteristics make them an excellent alternative to...