Immunoglobulins and Isotype Controls

Non-specific immunoglobulins or isotype controls are often used as standards or controls in antibody-based experiments. They include all proteins of an antibody class (eg. IgG or IgM ) or subclass (eg. IgG1 ) purified from a host or cell line. Isotype controls are used, for example, as negative controls in immunohistochemistry (IHC) and flow cytometry (FC, ​​FACS) where the properties should match those of the primary antibody used.

Non-specific immunoglobulins or isotype controls are often used as standards or controls in antibody-based experiments. They include all proteins of an antibody class (eg. IgG or IgM ) or subclass... read more »
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Immunoglobulins and Isotype Controls

Non-specific immunoglobulins or isotype controls are often used as standards or controls in antibody-based experiments. They include all proteins of an antibody class (eg. IgG or IgM ) or subclass (eg. IgG1 ) purified from a host or cell line. Isotype controls are used, for example, as negative controls in immunohistochemistry (IHC) and flow cytometry (FC, ​​FACS) where the properties should match those of the primary antibody used.

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Mouse IgA Kappa myeloma protein
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Human IgG F(ab')2 Fragment
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