Biochemicals

TRAP-6 peptide is a hexapeptide corresponding to residues 42-47 of protease-activated receptor 1 (PAR1). It acts as an agonist of PAR1, inducing platelet aggregation in human platelet-rich plasma ex vivo (EC50 = 0.8 µM). TRAP-6 (0.3 and 0.6 mg/kg) has a triphasic effect on mean arterial blood pressure (MAP) in anesthetized rats with a short decrease, an increase, and then a...

Purity: >96.5%, A reversible inhibitor of trypsin-like proteases and cysteine proteases. Soluble in water, ethanol, acetic acid and DMF. Stock solution: 10 mM in water, stable for 1 month at -20 °C. Effective conc.: 1-10 µM.

Soluble in DMF, hardly soluble in water. Use as alkaline phosphatase substrate forming a water insoluble blue reaction product. Often used together with NBT (Art.-Nr. 06428) for immunocytochemistry.

Assay: >85% enzymatically

Used in transfection studies.

Purity: >=99.5%. Suitable for electrophoresis

Purity: >99.9% (HPLC). Cell-permeable and irreversible inhibitor of calpain and other cystein proteases. Soluble in aqueous solutions. Stock solution: 1 mM, stable for months at -20 °C. Effective concentration: 1-10 µM.

Purity: >99%.

The water soluble MTT yields a yellowish solution. It is converted by mitochondrial dehydrogenases of metabolically active cells to an insoluble formazan product, which can be solubilized with DMSO. Read at 510 nm.

Cleland's reagent. Protective reagent for sulfhydryl groups of proteins and enzymes. maintains monothiols in the reduced state and reduces disulfides quantitatively.

Purity: >99.0%. Widely used as a buffer component in Laemmli gels and other applications.

Purity: >99%. Free of dioxane and other solvents. For use im molecular biology. In combination with X-Gal (cat# 02249) it is used to detect lac+ colonies in cloning experiments.