Anti-Protein Phosphatase 2 (formerly 2A), Catalytic Subunit, beta Isoform (Protein Phosphatase Type

Protein phosphatase type 2A (PP2A), an essential protein serine/threonine phosphatase conserved in all eukaryotes, regulates fundamental cellular activities such as DNA replication, transcription and translation, metabolism, cell cycle progression and cell division, apoptosis, development and various signal transduction pathways. The core enzyme consists of a catalytic C and a regulatory A (also known as PR65) subunit and each subunit exists as a and b isoforms. Another regulatory B subunit is composed of four different families of proteins with no sequence similarity between them. The B or PR55 family is composed of a, b, g and d isoforms, BI or PR61 family is composed of a, b, g, d and e isoforms, BII family consists of PR72, PR130, PR59 and PR48, BIII family has two members, striatin (PR110) and SG2NA (PR93). These B subunits bind to the same region of the A subunit and their interaction with the core enzyme is mutually exclusive. The diversity of the regulatory subunits, especially the B subunits, allows PP2A to perform its diverse functions. The function of PP2A is regulated by expression, localization, holoenzyme composition and post-translational modification. It has been reported that PP2A is phosphorylated at Tyr307 by Src in response to EGF or insulin, which inactivates PP2A activity. Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed. Methylation alters the conformation of PP2A, as well as its localization. PP2A methylation also regulates its association with B regulatory subunits. Applications: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:100, Immunohistochemistry (Paraffin): 1:100, Immunofluorescence (IF-IC): 1:50, Flow Cytometry: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.