Anti-Protein Phosphatase-2A, Serine, Threonine (PP2A Ser/Thr)

Anti-Protein Phosphatase-2A, Serine, Threonine (PP2A Ser/Thr)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
P9107-33A.200 200 µg - -

3 - 19 business days*

863.00€
 
Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially... more
Product information "Anti-Protein Phosphatase-2A, Serine, Threonine (PP2A Ser/Thr)"
Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases. Applications: Suitable for use in Western Blot, Immunoprecipitation and Phosphatase assays. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. Detects PP2A catalytic subunit (~34kD) in rabbit brain membrane cytosol and in human A431 RIPA cell lysate. , Immunoprecipitation: 4ug immunoprecipitates PP2A catalytic subunit from 500ug rabbit brain extract and 1mg human A431 RIPA cell lysate., Immunoprecipitation/Phosphatase Assay: 5ug immunoprecipitates PP2A from 1U of purified Protein Phosphatase 2A, Serine, Threonine (PP2A Ser/Thr). Phospatase activity was demonstrated using p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Ser/Thr Phosphatase Assay Kit, BioAssay(TM) (Catalog No: N2600-35)., Optimal dilutions to be detemined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: P9107-33A

Properties

Application: IP, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Species reactivity: human, mouse, rabbit, rat
Format: Affinity Purified

Database Information

KEGG ID : K04382 | Matching products
UniProt ID : P62714 | Matching products

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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