Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit

Detection principle: Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. The activity of mitochondrial complex V can be quantified by measure the change OD value at 340 nm. Performance characteristics: Synonyms: F0F1-ATPase, ATP Synthase. Sample type: cell. Sensitivity: 3.40 U/L. Detection range: 3.40-272.07 U/L. Detection method: Colorimetric method. Assay type: Enzyme Activity. Assay time: 30 min. Precision: Average inter-assay CV: 3.0-6.5%, Average intra-assay CV: 3.0-4.0%. Storage: -20?. Valid period: 12 months. Protein function: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel, it may play a direct role in the translocation of protons across the membrane. [The UniProt Consortium]