Cell Viability and Cytotoxicity Assays

The ScienCell(TM) Live/Dead Cell Staining Kit is a two-color fluorescence assay for convenient discrimination of live cells from dead cells. This kit can be applied to a fluorescence microscope, a fluorescent plate reader, or a flow cytometer. All cells, live and dead, will fluoresce green. Due to changes in...

Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme present in most eukaryotic cells. LDH releases into culture medium upon cell death due to damage of plasma membrane. The increase of the LDH activity in culture supernatant is proportional to the number of lysed cells. LDH Cytotoxicity Assay kit provides a...

The reduction of tetrazolim salts to colored formazan compounds by succinate-tetrazolim reductase provides a sensitive and accurate method to measure cell viability and proliferation. The cleavage of the tetrazolim salts to formazan takes place in the mitochondria of metabolically active cells. ScienCell's WST-1...

Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme present in most eukaryotic cells. LDH releases into culture medium upon cell death due to damage of plasma membrane. The increase of LDH activity in culture supernatant is proportional to the number of lysed cells. The LDH Cytotoxicity Assay Kit, Fluorescence...

Luminescence intensity is directly proportional to intracellular ATP (adenosine triphosphate), enabling a sensitive and reliable measurement of cell viability across multiple cell types. Because ATP is present only in metabolically active cells and rapidly declines during cell death, the luminescent output reflects...

The study of cell viability and proliferation is very important for evaluating a cell population's responses to external factors, such as growth factors, antibiotics and anti-cancer drugs. The ScienCellTM MTT Cell Viability & Proliferation Assay allows simple, accurate and reliable counting of metabolically active...

Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, while apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of...

Autophagy is a critical cellular process that involves the degradation and digestion of intracellular components by the lysosome. This process not only enables cells to efficiently mobilize and recycle cellular constituents, but also prevents the accumulation of damaged organelles, misfolded proteins, and invading...

Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. Cayman's XTT cell...