Reporter Assays

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Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence)
Secreted Alkaline Phosphatase Reporter Gene Assay Kit...

Item number: Cay600260-300

Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman's...
Keywords: SEAP Reporter Gene
Application: Chemiluminescence
287.00€ *
Review
One-Step Luciferase Assay System
One-Step Luciferase Assay System

Item number: BPS-60690-1

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component...
Application: Firefly luciferase assays, HTS
From 171.00€ *
Review
AR (human) Reporter Assay Kit
AR (human) Reporter Assay Kit

Item number: IB-IB03001

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO's reporter cells include the luciferase...
Keywords: AR, DHTR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Application: Functional activity screening
Reactivity: Human
From 1,121.00€ *
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Notch Reporter Assay Kit (cAMP/PKA pathway)
Notch Reporter Assay Kit (cAMP/PKA pathway)

Item number: BPS-60509

Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready Notch1 NICD expression vector and CSL (CBF1/RBP-Jkappa) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a...
Application: Notch activity assays
743.00€ *
Review
WNT TCF/LEF reporter kit
WNT TCF/LEF reporter kit

Item number: BPS-60500

The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / b-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway- responsive reporter. This reporter contains a firefly luciferase gene under the control of...
Keywords: LEF1, LEF-1, TCF1-alpha, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha reporter
Application: Wnt pathway activation assays, TCF/LEF reporter assay
Reactivity: Human
712.00€ *
Review
RORgamma (human) Reporter Assay Kit
RORgamma (human) Reporter Assay Kit

Item number: IB-IB04001

This human RAR-related orphan receptor gamma (RORgamma) mRNA is expressed from the RORC gene in two forms via alternate usage of tissue-specific promoters. Variant 1 mRNA is expressed in numerous tissues, and encodes receptor isoform 1, referred to as RORgamma. Variant 2 mRNA comprises an alternate exon 1 that...
Keywords: RORC, NR1F3, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, RAR-related orphan receptor C, Retinoid-related...
Application: Functional activity screening
Reactivity: Human
From 1,121.00€ *
Review
ERalpha (human) Reporter Assay Kit
ERalpha (human) Reporter Assay Kit

Item number: IB-IB00401

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 1 (NR3A1), a ligand-dependent transcription factor commonly referred to as ERalpha. INDIGO's reporter cells include the luciferase reporter gene...
Keywords: ER, ESR, ESR1, ER-alpha, Estrogen receptor, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
Application: Functional activity screening
Reactivity: Human
From 1,121.00€ *
Review
PXR (human) Reporter Assay Kit
PXR (human) Reporter Assay Kit

Item number: IB-IB07001

Protein function: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the...
Keywords: SXR, PXR, NR1I2, Pregnane X receptor, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, Steroid and xenobiotic...
Application: Functional activity screening
Reactivity: Human
From 1,121.00€ *
Review
ARE Reporter Kit (Nrf2 Antioxidant Pathway )
ARE Reporter Kit (Nrf2 Antioxidant Pathway )

Item number: BPS-60514

The ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, which is an Nrf2 pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ARE...
Keywords: NRF2, HEBP1, NFE2L2, NFE2-related factor 2, NF-E2-related factor 2, Nuclear factor, erythroid derived 2, like 2, Nuclear...
Application: Nrf2 pathway activity studies, Activator/inhibitor screening
743.00€ *
Review
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Prostaglandin F Receptor Reporter Assay Kit
Prostaglandin F Receptor Reporter Assay Kit

Item number: Cay601800-1

Prostaglandin F2alpha (PGF2alpha) is a biologically important prostanoid that exerts its actions through binding to the PGF2alpha receptor (FP). PGF2alpha is synthesized in many tissues, including the eye and organs of the female reproductive system. Activation of ocular FP receptors via agonists such as bimatoprost...
Keywords: PTGFR, PGF receptor, PGF2-alpha receptor, Prostanoid FP receptor, Prostaglandin F2-alpha receptor
Application: Luminescent reporter assay
500.00€ *
Review
RORa (Human) Reporter Assay System
RORa (Human) Reporter Assay System

Item number: IB-IB04011-32

Protein function: Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of embryonic development, cellular differentiation, immunity, circadian rhythm as well as lipid, steroid,...
Keywords: RORA, NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A, Retinoid-related...
Application: Functional activity screening
Reactivity: Human
From 1,121.00€ *
Review
SRE Reporter Kit (MAPK/ERK Signaling Pathway )
SRE Reporter Kit (MAPK/ERK Signaling Pathway )

Item number: BPS-60511

The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus...
Application: MAPK/ERK signaling pathway assays
712.00€ *
Review
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