Reporter Assays

Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman's...

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component...

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO's reporter cells include the luciferase...

Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready Notch1 NICD expression vector and CSL (CBF1/RBP-Jkappa) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a...

This human RAR-related orphan receptor gamma (RORgamma) mRNA is expressed from the RORC gene in two forms via alternate usage of tissue-specific promoters. Variant 1 mRNA is expressed in numerous tissues, and encodes receptor isoform 1, referred to as RORgamma. Variant 2 mRNA comprises an alternate exon 1 that...

Protein function: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the...

Prostaglandin F2alpha (PGF2alpha) is a biologically important prostanoid that exerts its actions through binding to the PGF2alpha receptor (FP). PGF2alpha is synthesized in many tissues, including the eye and organs of the female reproductive system. Activation of ocular FP receptors via agonists such as bimatoprost...

Protein function: Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of embryonic development, cellular differentiation, immunity, circadian rhythm as well as lipid, steroid,...

Early screening of a drug candidate's potential to induce or inhibit CYP2B6 is a critical step in the drug development process. Cayman's CYP2B6 Induction Reporter Assay Kit is a novel method to evaluate human Constitutive Androstane Receptor 3 (hCAR3)-mediated CYP2B6 induction for the discovery of hepatic CYP...

Bile acids are a diverse class of amphipathic molecules originally formed in liver and modified by microorganisms in the intestine. In addition to their conventional role in dietary lipid absorption, bile acids have been recognized as signaling molecules that regulate the synthesis and metabolism of bile acids,...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive high-level expression of full-length, unmodified human mineralocorticoid receptor (NR3C2), a ligand-dependent transcription factor commonly referred to as MR. INDIGO's reporter cells include the luciferase...

This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the full-length human progesterone receptor (NR3C3), a ligand-dependent transcription factor commonly referred to as PR. INDIGO's reporter cells include the luciferase reporter gene...