Reporter Assays

Prostaglandin F2alpha (PGF2alpha) is a biologically important prostanoid that exerts its actions through binding to the PGF2alpha receptor (FP). PGF2alpha is synthesized in many tissues, including the eye and organs of the female reproductive system. Activation of ocular FP receptors via agonists such as bimatoprost...

The Melanocortin-3 receptor (MC3R) helps regulate energy homeostasis and mice lacking MC3R have increased fat mass and reduced lean mass. Therefore, agonists that selectively activate MC3R might have beneficial effects related to weight gain and glucose metabolism. This assay consists of a 96-well plate coated with...

The melanocortin-4 receptor (MC4R) has important roles in weight regulation, sexual function, and inflammation. Mice deficient in MC4R have increased lipid deposition associated with elevated adiposity, while mutations in MC4R in humans are associated with early onset or severe obesity. This assay consists of a...

Surface Transfection and Expression Protocol (STEP(TM)) is a novel, patented, solid phase transient transfection technology that overcomes many of the disadvantages of other transfection approaches. CaymansOrexin 1 Receptor (OX1R) STEP(TM) Reporter Assay Kit (Luminescence) consists of a 96-well plate coated with...

Surface Transfection and Expression Protocol (STEP(TM)) is a novel, patented, solid phase transient transfection technology that overcomes many of the disadvantages of other transfection approaches. CaymansOrexin 2 Receptor (OX2R) STEP(TM) Reporter Assay Kit (Luminescence) consists of a 96-well plate coated with...

Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman's...

The EP2 receptor is one of four GPCRs that mediate the actions of PGE2. The diverse effects of PGE2 acting via EP2 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP2...

The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various...

Early screening of a drug candidate's potential to induce or inhibit CYP1A1/2 is a critical step in the drug development process. Cayman's CYP1A1/2 Induction Reporter Assay Kit is a novel method to assess CYP1A2 induction. Our Reverse Transfection Reporter Assays have overcome many of the disadvantages of other...

Early screening of a drug candidate's potential to induce or inhibit CYP2B6 is a critical step in the drug development process. Cayman's CYP2B6 Induction Reporter Assay Kit is a novel method to evaluate human Constitutive Androstane Receptor 3 (hCAR3)-mediated CYP2B6 induction for the discovery of hepatic CYP...

Early screening of drug candidate's potential to induce or inhibit CYP2C9 is a critical step in the drug development process. The CYP2C9 Induction Reporter Assay Kit is a novel method to evaluate CYP2C9 induction by drug candidates. Cayman's CYP2C9 Induction Reporter Assay Kit consists of a 96-well plate coated with...

Free fatty acid receptor 1 (FFAR1)/GPR40 is a G protein-coupled receptor for long-chain fatty acids such as docosahexaenoic acid and eicosapentaenoic acid omega-3 fatty acids. It is involved in the regulation of insulin release from pancreatic beta cells and mediates triglyceride-induced secretion of incretins GLP-1...