Reporter Assays

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component...

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO's reporter cells include the luciferase...

This human RAR-related orphan receptor gamma (RORgamma) mRNA is expressed from the RORC gene in two forms via alternate usage of tissue-specific promoters. Variant 1 mRNA is expressed in numerous tissues, and encodes receptor isoform 1, referred to as RORgamma. Variant 2 mRNA comprises an alternate exon 1 that...

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist. This kit product is an all-inclusive assay system that includes, in addition to Reporter Cells, two optimized media for use during cell culture and (optionally) in...

The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / b-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway- responsive reporter. This reporter contains a firefly luciferase gene under the control of...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive high-level expression of full-length, unmodified human mineralocorticoid receptor (NR3C2), a ligand-dependent transcription factor commonly referred to as MR. INDIGO's reporter cells include the luciferase...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human peroxisome proliferator-activated receptor gamma (NR1C3), a ligand-dependent transcription factor commonly referred to as PPARG or PPARgamma. INDIGO's reporter cells include...

Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready Notch1 NICD expression vector and CSL (CBF1/RBP-Jkappa) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a...

Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman's...

Cayman's reverse transfection reporter assays have overcome many of the disadvantages of other transfection approaches. In this method, a proprietary transfection complex containing DNA and an optimized mixture of lipids and proteins has been evenly applied and processed on the culture surface of multi-well plates....

Free fatty acid receptor 1 (FFAR1)/GPR40 is a G protein-coupled receptor for long-chain fatty acids such as docosahexaenoic acid and eicosapentaenoic acid omega-3 fatty acids. It is involved in the regulation of insulin release from pancreatic beta cells and mediates triglyceride-induced secretion of incretins GLP-1...