Other Cell-Based Assays

The proteasome is a multicatalytic proteinase complex that is involved in the selective degradation of intracellular proteins. The 20S proteasome is the proteolytic core particle of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects,...

Cayman's Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell...

Glucose metabolism is a primary source of energy and biomaterials for the maintenance of cell homeostasis. Extra glucose is stored in the muscles and liver as glycogen which is hydrolyzed to glucose and released into the blood when needed. The rate of glucose uptake in cells is dynamic and tightly regulated by...

Cayman's MTT Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells through the plasma...

This kit is based on a cell-permeant fluorescent probe (MR-(RR)2) designed for real-time detection of cathepsin B activity within whole living cells, using a fluorescence microscope. Excitation wavelength 560nm, emission 610-650nm.

The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). Our Cell Navigator(TM) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as...

The oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells. Oxygen consumption is...

Cayman's WST-8 Cell Proliferation Assay provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of WST-8 by NADH produced in the mitochondria resulting in a water-soluble formazan which dissolves directly into the culture...

Cayman's Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a...

Extinction coefficient (cm^-1 M^-1): 250000 Excitation (nm): 651 Emission (nm): 670 Quantum yield: 0.27 (PBS), 0.4 (ethanol). Glucose metabolism is a primary source of cellular energy and biomaterials for maintaining cell homeostasis. Surplus glucose is stored as glycogen in muscular and hepatic tissues, with...

Detection principle: This kit provides a fluorescent probe that can specifically bind to Fe2+, and the probe can enter the cell interior well, which is suitable for the detection of Fe2+ in alive cells. When the probe reacts with Fe2+, an irreversible orange (red) fluorescent product is generated (excitation...

Detection principle: GPX catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents. The reducing agent has a maximum absorbance at 340 nm. The activity of GPX can be calculated by measuring the change of absorbance value at 340 nm. Performance characteristics: Sample...