Other Cell-Based Assays

The proteasome is a multicatalytic proteinase complex that is involved in the selective degradation of intracellular proteins. The 20S proteasome is the proteolytic core particle of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects,...

Cayman's Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell...

Glucose metabolism is a primary source of energy and biomaterials for the maintenance of cell homeostasis. Extra glucose is stored in the muscles and liver as glycogen which is hydrolyzed to glucose and released into the blood when needed. The rate of glucose uptake in cells is dynamic and tightly regulated by...

This kit is based on a cell-permeant fluorescent probe (MR-(RR)2) designed for real-time detection of cathepsin B activity within whole living cells, using a fluorescence microscope. Excitation wavelength 560nm, emission 610-650nm.

The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). Our Cell Navigator(TM) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as...

Cayman's WST-8 Cell Proliferation Assay provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of WST-8 by NADH produced in the mitochondria resulting in a water-soluble formazan which dissolves directly into the culture...

Cayman's Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a...

Cayman's Neutrophil Elastase Activity Assay kit employs a specific non-fluorescent elastase substrate, (Z-Ala-Ala-Ala-Ala)2Rh110, which is selectively cleaved by elastase to yield the highly fluorescent compound R110. Fluorescence can be analyzed with an excitation wavelength of 485 nm and emission wavelength of 525...

Cayman's Extracellular Oxygen Consumption Assay Kit uses a phosphorescent oxygen-sensitive probe. Because of its inability to cross the membrane, the probe selectively reports on changes in oxygen levels in the medium. The probe's signal is quenched upon binding to oxygen, resulting in a signal that is inversely...

Cayman's Adipolysis Assay Kit provides a complete assay for studying the lipolysis of triglycerides, from the differentiation of adipocyte-like cells to the quantitation of released glycerol. 3T3-L1 cells are differentiated using a common adipogenesis induction procedure to accumulate lipid droplets, and...

This kit is based on a cell-permeant fluorescent probe (MR-(FR)2) designed for real-time detection of cathepsin L activity within whole living cells, using a fluorescence microscope. Excitation wavelength 560nm, emission 610-650nm.

Cayman's Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell...