Cell Biology Assays

P-Glycoprotein (P-gp), the product of the multidrug resistance (MDR) gene MDR1, and multidrug resistance associated proteins (MRP) are members of the superfamiliy of ATP-binding cassette (ABC) transporters. They are localized in the luminal membrane of endothelial cells and function to detoxify or eliminate a spectrum of harmful agents and chemicals by mediating...

Cayman's Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating polypeptide chains, thereby stopping translation. These truncated C-terminal alkyne-labeled...

Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of reactive oxygen species (ROS) to form ethidium (E+) (ex 480 nm/em 576 nm). Given their narrow spectral...

Ex: 640 nm. Em: 659 nm. Our Cell NavigatorT fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed...

Ex: 639 nm. Em: 660 nm. Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative...

Ex: 556 nm. Em: 566 nm. Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative...

Fatty acid uptake is an important therapeutic target for the treatment of many human diseases such as obesity, type 2 diabete and hepatic steatosis. The ScreenQuest(TM) Fluorimetric Fatty Acid Uptake Assay Kit provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. The kit uses a proprietary dodecanoic acid...

Lysosomes are intracellular organelles that contain enzymes used for the hydrolysis of waste materials and other cellular debris. The pH of the lysosome is ~4.5-4.8 and is optimal for these hydrolytic enzymes. The lysosomal pH gradient is maintained through vacuolar ATPases which pump protons into the lysosomes. Cayman's Lysosomal Staining Kit (Red Fluorescence) utilizes a...

In the past, most cell-proliferation studies have used radioactive thymidine as an incorporated label to give evidence of DNA replication. Recently, a less hazardous technique has been developed using bromodeoxyuridine (BrdU), a thymidine analog, in lieu of a radioactive reagent. (1) BrdU is incorporated into proliferating cells (S-phase) much in the same way as radioactive...

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS). This process can lead to not only disruption of the cellular membrane integrity, but also inactivation of membrane-bound receptors. It is one of the main causes of free radical-mediated damages in cells. Cell Meter(TM) Fluorimetric Cellular Lipid Peroxidation Assay Kit...