Apoptosis and Cell Cycle Assays

Kit includes 660-YVAD-FMK red caspase-1 probe, 10x Apoptosis Wash Buffer, Fixative, Technical Protocol. The red FLICA® 660-YVAD-FMK caspase-1 detection probe is comprised of the preferred affinity peptide sequence for active caspase-1 (YVAD), a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK)...

Mitochondrial membrane potential, (DeltapsiM), is an important parameter of mitochondrial function used as an indicator of cell health. JC-1 is a lipophilic, cationic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells...

This kit uses Z-DEVD-Rh 110-DVED-Z as a fluorogenic indicator for caspase-3 activity. Using 100µl of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 100 assays.

This kits makes use of a cell-permeant green fluorecent probe (FAM-YVAD-FMK) that binds to active caspase-1 in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.

This kits makes use of a cell-permeant green fluorecent probe (FAM-YVAD-FMK) that binds to active caspase-1 in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.

The far-red FLICA® 660-DEVD-FMK caspase-3 detection probe is comprised of the preferred affinity peptide sequence (DEVD) targeted by activated caspase-3 and caspase-7, a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting fluorescent caspase-3 inhibitor probe forms an...

DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that...

Although JC-1 is widely used in many labs, its poor water solubility causes extraordinary inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, JC-10 has much better...

Our Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for...

This kit uses Ac-DEVD-AMC as a fluorogenic indicator for caspase-3 activity. Sufficient reagents to perform 500 assays. Using 25µl of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 2,000 assays.

Designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). The PS sensor used in this kit is a single fluorophore-labeled Annexin V that is specially modified to bind PS more tightly.

Cell Meter(TM) TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation. The assay is non-radioactive and rapid. The TUNEL assay uses the terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of TF5-dUTP at the free 3'-hydroxyl ends of the...