Apoptosis and Cell Cycle Assays

One of the hallmarks of the early stages of apoptosis is the redistribution of membrane phospholipids such as phosphatidylserine and phosphatidylethanolamine from the inner to outer leaflet of the membrane bilayer where they are exposed on the cell surface. Externalization of phosphatidylserine residues to the outer plasma membrane leaflet allows their detection via their...

Cayman's Early Apoptosis Detection Assay Kit employs FITC-conjugated annexin V as a probe for phosphatidylserine (PS) on the outer membrane of apoptotic cells, TMRE as a probe for DeltaPsim, DAPI as an indicator of membrane permeability/cell viability, and TO-PRO(R)-3 to indicate the selective permeability of pannexin channels. The Early Apoptosis Detection Assay Kit allows...

Apoptosis or programmed cell death is an evolutionary conserved, intrinsic program of cell death that occurs in various physiological and pathological situations. It is a key regulator of physiological growth control and regulation of tissue homeostasis. Apoptosis is considered a vital component of various processes including normal cell turnover, proper development and...

This kits makes use of a cell-permeant green fluorecent probe (FAM-VAD-FMK ) that binds to all active caspases in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.

This kits makes use of a cell-permeant green fluorecent probe (FAM-LETD-FMK ) that binds to active caspase-8 in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.

MitoPT(TM)-TMRE Depolarization Detection Kits can evaluate apoptosis using flow cytometry, fluorescence microscopy, or a fluorometric plate reader.

MitoPT(TM)-TMRM Depolarization Detection Kits can evaluate apoptosis using flow cytometry, fluorescence microscopy, or a fluorometric plate reader.

Mitochondrial Permeability Transition Kit that allows the easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells. It detects mitochondrial membrane breakdown, associated with cytochrome C release.

This kits makes use of a cell-permeant green fluorecent probe (FAM-LEHD-FMK ) that binds to active caspase-9 in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.

The red FLICA® 660-VAD-FMK caspase detection probe is comprised of the preferred affinity peptide sequence (VAD) targeted by activated pan-caspases, a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting fluorescent caspase inhibitor probe forms an irreversible, covalent bond with active caspase enzymes, efficiently labeling the...

Kit includes 660-YVAD-FMK red caspase-1 probe, 10x Apoptosis Wash Buffer, Fixative, Technical Protocol. The red FLICA® 660-YVAD-FMK caspase-1 detection probe is comprised of the preferred affinity peptide sequence for active caspase-1 (YVAD), a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting probe forms an irreversible,...