Cell Meter(TM) Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit

Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. Malondialdehyde (MDA) is one of the most commonly used biomarkers for lipid peroxidation. Measurement of MDA has historically relied on a reaction with thiobarbituric acid (TBA) to generate a product that can be measured colorimetrically at 532 nm or fluorimetrically at Ex/Em = 530550 nm. However, TBA assay has quite a few limitations. (1). The reaction is not specific to MDA. (2). TBA-MDA reaction need be run under acidic conditions. (3). The assays need be run under high temperature, commonly at 90-100 ºC. The commercial TBA-based MDA assays are extremely tedious to run due to these limitations. This Cell Meter(TM) Colorimetric Lipid Peroxidation (MDA) Quantitation Kit offers the most rapid and convenient method to measure MDA without the TBARS heating steps. MDA Blue(TM) reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate reader. This assay is very fast and specific to MDA with little interference from other aldehydes.