Other Cell-Based Assays

Cayman's Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets. Furthermore, changes in lipid droplet biogenesis in various cell types in...

Nucleosomes, which fold chromosomal DNA, contain two molecules each of the core histones H2A, H2B, H3, and H4. Almost two turns of DNA are wrapped around this octameric core, which represses transcription. The histone amino termini extend from the core, where they can be modified post-translationally by acetylation,...

Chloride channels have a variety of important physiological and cellular functions that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Chloride channels represent valuable drug targets. A number of chronic disease states such as cystic...

The mechanism for the movement of cholesterol from intracellular sites to their ultimate cellular destination is an unresolved question of fundamental importance to cell biology and medicine. Thus, defining mechanisms of intracellular cholesterol transport and identifying the cellular factors involved are therefore...

This particular kit is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for relatively longer time.

Efferocytosis is a specialized form of phagocytosis in which macrophages and other phagocytic cells clear dead and dying cells to promote homeostasis, embryonic development, regulation of the immune system and the resolution of inflammation. Efficient clearance of cells is essential for the removal of the nearly 1...

In mammals, triglycerides are constantly synthesized from fatty acids and segregated into cytosolic lipid droplets, mainly in adipocytes, as the major energy storage depot. During fasting, triglycerides stored in adipose tissue and liver are hydrolyzed by hormone-sensitive lipase and adipose triglyceride lipase to...

Quantitate and monitor intracellular cathepsin K activity over time in vitro. The Magic Red reagent in this assay fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescent plate reader. This kit is based on a cell-permeant fluorescent...

The kit uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably vial the lysosome pH gradient. The dye easily permeates intact live cells, and trapped in lysosomes after it gets into cells.

Cayman's E. coli Phagocytosis Assay Kit employs FITC-labeled, heat-inactivated E. coli cells for the measurement of the phagocytic process in vitro. The engulfed fluorescent E. coli can be detected by flow cytometry.Origin: Animal/Bovine.

Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative...

Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative...