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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
E-CK-A345.500 | 500 assays | - | - |
7 - 16 business days* |
148.00€
|
||
E-CK-A345.2000 | 2000 assays | - | - |
7 - 16 business days* |
446.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Detection principle: CFDA SE is a kind of fluorescein dye with membrane permeability, which has... more
Product information "CFSE Cell Division Tracker Kit"
Detection principle: CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps. The fluorescence of CFDA SE-labeled cells is uniform and stable. In the process of cell division and proliferation, the fluorescence of CFDA SE-labeled cells could be evenly distributed to two progeny cells, and the fluorescence intensity becomes half of the parental cells. According to the difference of fluorescence intensity, the detection results can be divided into undivided cells, cells that have split once (1/2 fluorescence intensity), cells that have split twice (1/4 fluorescence intensity), cells that have split three times (1/8 fluorescence intensity), and cells that have split more times. CFDA SE-labeled cells can be used for in vitro and in vivo proliferation studies without staining of neighboring cells. CFDA SE-labeled cells showed green fluorescence, Ex=494 nm, Em=521 nm, which can be detected by flow cytometry or fluorescence microscope. General Information: Application: Cell Proliferation. Detection method: Fluorometric Method. Sample type: Cell samples. Detection instrument: Flow Cytometry, Fluorescence Microscope. Dye Type: CFSE Ex/Em (nm):: 494/521. Channel set: FITC. Filter set: FITC. Other reagents required: PBS (pH7.2~7.6) (E-BC-R187). Storage: -20°C, shading light. Shipping: Ice bag. Expiration date: 12 months.
Supplier: | Elabscience |
Supplier-Nr: | E-CK-A345 |
Properties
Application: | in vitro/in vivo proliferation studies, FC, FM |
Species reactivity: | broad |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | -20°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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