Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
BOT-30072 | 1 set (100 assays) | - |
Request delivery time estimate |
534.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
NucView(R)488 and RedDot(TM) Apoptosis/Necrosis Assay Kit contains NucView(R)488 Caspase-3... more
Product information "NucView(R) 488 and RedDot(TM) 2 Apoptosis & Necrosis Kit"
NucView(R)488 and RedDot(TM) Apoptosis/Necrosis Assay Kit contains NucView(R)488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot(TM) is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView(R)488 Caspase-3 Substrate can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView(R)488 Caspase-3 Substrate allows detection caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. RedDot(TM) is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. RedDot(TM) has far-red emission at 695 nm for detection in the Cy(R) channel, well-separated from the green fluorescence of NucView(R)488. The excitation maximum of RedDot(TM) is 665 nm, but it can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line. Note: while NucView(R)488 staining is formaldehyde-fixable and compatible with subsequent immunostaining, fixation after staining with RedDot(TM) is not recommended because it can result in increased background staining of healthy cells. See our full selection of NucView(R) substrates and kits. NucView(R) enzyme substrate technology is covered by U.S. patents. Cy5 is a registered trademark of GE Healthcare.
Supplier: | Biotium |
Supplier-Nr: | 30072 |
Properties
Database Information
Handling & Safety
Storage: | +4°C |
Shipping: | +20°C (International: +20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
Viewed