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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
A1124-90A.100 | 100 µg | - | - |
3 - 19 business days* |
863.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved... more
Product information "Anti-phospho-Akt, (Ser473) (Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thym"
Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including glucose metabolism, cell survival/apoptosis, cell cycle control, angiogenesis, differentiation and cell growth and proliferation. In mammals three isoforms of Akt (Akt1/PKB alpha, Akt2/PKB beta, and Akt3/PKB gamma) exist. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin- responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module to direct Akt to PIP3 at the cell membrane that is necessary for its activation, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of Akt is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 2ug/ml, Flow Cytometry: 1:800, Western Blot: 0.5-1ug/ml detected phosphorylated AKT in lysates from mouse NIH-3T3 fibroblasts treated with 100ng/ml PDGF for 20 minutes., Immunohistochemistry: 1:200, Pretreatment with citrate, pH 6 antigen retrieval., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | EC=2.7.11.1, Anti-phospho-PKB, Anti-phospho-AKT1, Anti-phospho-PKB alpha, Anti-phospho-RAC-PK-alpha, Anti-phospho-Proto-oncogene c-Akt, Anti-phospho-Protein kinase B alpha |
Supplier: | United States Biological |
Supplier-Nr: | A1124-90A |
Properties
Application: | ELISA, FC, IF, IHC, WB |
Antibody Type: | Monoclonal |
Clone: | 9i145 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human, mouse, rat |
Immunogen: | Peptide encompassing and including phosphorylated Ser473 of human Akt1. |
Format: | Affinity Purified |
Database Information
KEGG ID : | K04456 | Matching products |
UniProt ID : | P31749 | Matching products |
Gene ID | GeneID 207 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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