Anti-p38alpha MAPK (Phospho-Thr180/Tyr182) Rabbit Monoclonal Antibody (Clone RM243)

p38 MAPK is a serine/threonine protein kinase and member of the MAPK family with roles in the regulation of immune responses and embryonic development, as well as cell differentiation, metabolism, and survival. It exists as 4 isoforms, p38alpha, -beta, -gamma, and -delta, encoded by MAPK14, MAPK11, MAPK12, and MAPK13, respectively, in humans. p38alpha MAPK is ubiquitously expressed, with the highest levels of expression in the heart, skeletal muscle, and brain. It is activated via dual phosphorylation of threonine 180 (Thr180) and tyrosine 182 (Tyr182) by the MAP2K kinases MKK3 and MKK6 in response to LPS or the production of inflammatory cytokines and induces signaling through protein kinases, transcription factors, and transcriptional regulators, among others. Levels of activated p38alpha MAPK (p38alpha phospho-Thr180/Tyr182) are increased and positively correlated with apoptosis in DU145 and PC3 prostate cancer cells in response to cisplatin (Cay-13119). p38alpha Phospho-Thr180/Tyr182 levels are also increased in adult rat ventricular monocytes during stimulated ischemia. Cayman's p38alpha MAPK (Phospho-Thr180/Tyr182) Rabbit Monoclonal Antibody (Clone RM243) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes p38alpha MAPK (phospho-Thr180/Tyr182) at approximately 38 kDa from human samples.Synonyms: MAPK14 (Phospho-Thr180/Tyr182), Mitogen-activated Protein Kinase 14 (Phospho-Thr180/Tyr182), Mitogen-activated Protein Kinase p38alpha (Phospho-Thr180/Tyr182), Stress-activated Protein Kinase 2A (Phospho-Thr180/Tyr182). Immunogen: Peptide corresponding to human p38alpha MAPK (phospho-Thr180/Tyr182). Clone Designation: RM243. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) p38alpha MAPK (phospho-Thr180/Tyr182), (-) Unmodified p38alpha MAPK, (-) p38alpha MAPK (phospho-Thr180), (-) p38alpha MAPK (phospho-Tyr182).