Anti-Topoisomerase II alpha / TOP2A, clone rTOP2A/8339

1 mg/ml in 1X PBS, BSA free, sodium azide free. It recognizes a 170kDa protein, which is identified as topoisomerase IIAlpha. It shows no cross-reaction with Topoisomerase II beta or I. Topo II Alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II Alpha is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents. Protein function: Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation. [The UniProt Consortium]