Anti-Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase, TANK2, TNKL)

The 3'ends of chromosomes are capped with telomere sequences (TTAGGG, 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling. Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate. Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity. Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase, human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers. TANK1 has 24 ankyrin repeats in TRF-1 interacting domain near the N-terminus. The ANK domain is followed another protein interaction motif called the sterile alpha-module (SAM). The C-terminal region of TANK1 contains the PARP activity. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000., ELISA: 1:10,000-50,000. T0900-08 Control peptide can be used to coat ELISA plates at 1ug/ml., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.