Anti-Tankyrase 2 (TNKS2, Tankyrase, TRF1-interacting Ankyrin-related ADP-ribose polymerase 2, PARP-5

Tankyrase (TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyase 1, TANK1, TNKS1) is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (1). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase colocalizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes. Tankyrase 2 (TRF1-interacting akyrin-related ADP-ribose polymerase 2, TANK2, TNKS2) is a closely related homolog of tankyrase 1. Tankyrase 2 lacks the N-terminus HPS domain (containing homo-polymeric runs of histidine, proline, and serine) found in tankyrase 1. Applications: Suitable for use in Peptide ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:16,000, Western Blot: 0.5-1.5ug/ml. Observed band at ~125kD. An additional band of unknown identity was observed at ~45kD., Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell line lysates, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.