Anti-Smac (C-Term) Rabbit Monoclonal Antibody (Clone RM271)

Smac is a mitochondrial protein involved in the initiation of apoptosis. It is a ubiquitously expressed alpha-helical protein that forms homodimers. It is localized to the mitochondria but is released following apoptotic stimuli into the cytoplasm, where it binds to inhibitor of apoptosis proteins (IAPs), which are overexpressed in a variety of cancer cells. Smac binding to IAPs blocks their inhibitory effect on caspase-3, -7, and -9 and leads to the activation of apoptosis. Knockout of DIABLO, the gene encoding Smac, in colon cancer cells in vitro induces resistance to apoptosis induced by NSAIDs and TRAIL, but not cisplatin (Cay-13119), mitomycin C (Cay-11435), or camptothecin (Cay-11694). DIABLO knockout in mice does not induce changes in apoptosis or tumorigenesis, indicating a redundancy of its function. Overexpression of DIABLO in vitro does not affect basal levels of apoptosis but increases the sensitivity of cells to other apoptotic stimuli. Cayman's Smac Rabbit Monoclonal Antibody (Clone RM271) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: DIABLO, Diablo Homolog, Mitochondrial, Direct IAP-binding Protein with Low pI, Second Mitochondria-derived Activator of Caspase. Immunogen: Peptide from the C-terminal region of human Smac. Clone Designation: RM271. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC and WB. Cross Reactivity: (+) Smac.