Anti-Smac / Diablo

0.5mg/ml if reconstituted with 0.2ml sterile DI water. Smac antibody targets Second mitochondria-derived activator of caspases, commonly known as Smac or Diablo, encoded by the DIABLO gene. Smac is a mitochondrial protein that plays a critical role in the intrinsic apoptotic pathway by promoting caspase activation. Under normal conditions, Smac is localized within the mitochondrial intermembrane space. Upon apoptotic stimulation, it is released into the cytosol, where it functions as a pro-apoptotic regulator by counteracting inhibitor of apoptosis proteins (IAPs).Functionally, Smac enhances apoptosis by binding to IAP family members such as XIAP, cIAP1, and cIAP2, thereby preventing these proteins from suppressing caspase activity. Through this mechanism, Smac facilitates the activation of effector caspases and promotes efficient execution of programmed cell death. This role positions Smac as an important checkpoint protein that helps determine whether apoptotic signals proceed to irreversible cell death. A Smac antibody supports studies focused on mitochondrial apoptosis and caspase regulation.DIABLO is broadly expressed across tissues, reflecting the universal requirement for tightly regulated apoptotic machinery in multicellular organisms. While Smac is constitutively present in mitochondria, its cytosolic activity is strictly regulated and occurs primarily in response to cellular stress, DNA damage, or death receptor signaling. The controlled release of Smac ensures that apoptosis is activated only under appropriate conditions, maintaining tissue homeostasis and preventing unintended cell loss.From a disease-relevance perspective, dysregulation of Smac-mediated apoptosis has been implicated in cancer, neurodegenerative disease, and immune disorders. Reduced Smac expression or impaired release from mitochondria can contribute to apoptosis resistance in tumor cells, supporting uncontrolled survival and therapy resistance. Conversely, excessive or inappropriate activation of apoptotic pathways involving Smac has been studied in degenerative conditions characterized by unwanted cell death. These findings have made Smac a molecule of interest in studies of apoptosis regulation and therapeutic targeting of cell death pathways.At the molecular level, Smac undergoes mitochondrial processing to generate its mature, active form, and post-translational regulation can influence its stability and interactions with IAP proteins. These factors can contribute to variable electrophoretic behavior on SDS-PAGE without implying changes in primary sequence. A Smac antibody supports research applications focused on apoptosis signaling, mitochondrial protein dynamics, and disease-associated alterations in cell death regulation, with NSJ Bioreagents providing reagents intended for research use.