Anti-PolD1

Anti-PolD1
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A304-007A-T 20 µl (2 blots) -

3 - 9 business days*

131.00€
A304-007A-M 100 µl (10 blots) -

3 - 9 business days*

313.00€
A304-007A 100 µl (100 µg) -

3 - 9 business days*

465.00€
 
Protein function: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric... more
Product information "Anti-PolD1"
Protein function: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). [The UniProt Consortium]
Keywords: Anti-POLD, Anti-POLD1, EC=2.7.7.7, EC=3.1.11.-, Anti-DNA polymerase subunit delta p125, Anti-DNA polymerase delta catalytic subunit
Supplier: Bethyl Laboratories
Supplier-Nr: A304-007A

Properties

Application: WB, IP
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human, Mouse (Expected: Rat, Bovine, Golden Hamster)
Immunogen: synthetic peptide. The epitope recognized by A304-007A-T maps to a region between residue 930 to 980 of human Polymerase (DNA directed), Delta 1, Catalytic Subunit 125kDa using the numbering given in entry NP_002682.2 (GeneID 5424).
Format: Antigen Affinity Purified

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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