Anti-Plexin A4 (PLXNA4, KIAA1550, PLXNA4A, PLXNA4B, UNQ2820/PRO34003) (PE)

Plexin A4 is a 220-230kD member of the plexin A subfamily of plexin family proteins. It is found on sensory, autonomic, and motor neurons, oligodendrocytes, T cells, and dendritic cells. Plexin A4 regulates cell migration, activation, and axon guidance via repulsion. It serves as a receptor for transmembrane semaphorins, Semaphorin 6A and 6B, and as a co-receptor with Neuropilin-1 for the secreted semaphorin, Semaphorin 3A. During development, it plays a role in nerve migration and midline crossing and down regulates dendrite formation. It is often co-expressed with Plexin A3, which can also engage class 6 semaphorins, but prefers Semaphorin 3F/Neuropilin-2 to Semaphorin 3A/Neuropilin-1. Thus, Plexins A3 and A4 are redundant in some functions, but unique in others. In T cells, Plexin A4 engages Semaphorin 3A and negatively regulates TCR signals. The extracellular domain of human Plexin A4 shares 97% aa identity with mouse, equine, canine, and bovine Plexin A4. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells, Optimal dilutions to be determined by the researcher. Excitation: 488nm , Emission: 565-605nm, Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.