Anti-Platelet Derived Growth Factor Receptor Type A (Platelet-derived Growth Factor Receptor alpha,

The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor a (PDGFRa) and PDGF receptor B (PDGFRb). PDGFra and PDGFRabbit share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology. PDGF Receptor A homodimers bind all PDGF isoforms except those containing PDGF D. PDGF Receptor B homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGFa/b receptor binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer. PDGFRa and PDGFRb can each form heterodimers with EGFR, which is also activated by PDGF. Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding. Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCy and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration and differentiation. Tyr751 in the kinase-insert region of PDGFRb is the docking site for PI3 kinase. Phosphorylated pentapeptides derived from Tyr751 of PDGFRb (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRb. Tyr740 is also required for PDGFRb mediated PI3 kinase activation. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IF-IC): 1:800, Flow Cytometry: 1:800, Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunohistochemistry (Paraffin): 1:200. Use EDTA as unmasking buffer., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.