Anti-Niemann-Pick Disease, Type C1 Gene-Like 1 (NPC1L1, Niemann-Pick C1-like Protein 1, NPC1 like 1,

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 detects a band at ~140kD representing NPC1L1 and a non-specific band may be observed at ~45kD depending on the lysate used., Immunofluorescence (IC): 1:100-1:250, Immunohistochemistry (Paraffin): 1:100-1:250, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.