Anti-Muscarinic Receptor M2, aa225-356 (Cholinergic Muscarinic Receptor M2, Chrm2, Muscarinic Recept

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1:200 (Rat brain membranes), Immunohistochemistry: Rat brain sections, Optimal dilutions to be determined by the researcher. Control Antigen Included: M9520-11A, Epitope: VANQD PVSPS LVQGR IVKPN NNNMP SSDDG LEHNK IQNGK APRDP VTENC VQGEE KESSN DSTSV SAVAS NMRDD EITQD ENTVS TSLGH SKDEN SKQTC IRIGT KTPKS DSCTP TNTTV EVVGS SGQNG DE, Epitope location: 3rd intracellular loop, Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.