101 products were found matching "K04130"!

For immunohistology, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's...

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C...

Recombinant CHO-K1 cells stably overexpress human cholinergic receptor muscarinic 2 (M2) on the surface and contain high levels of G protein Galphai to couple with the receptor in downstream signaling pathways.

Arecoline is a natural alkaloid that is found in the betel nut of the Areca palm. It is an agonist of the muscarinic acetylcholine receptors with EC50 values of 7, 95, 11, 410, and 69 nM for M1, M2, M3, M4, and M5, respectively. Generally, arecoline causes smooth muscle contraction. Arecoline and other muscarinic...

Atropine is a naturally occurring tropane alkaloid extracted from plants of the family Solanaceae including deadly nightshade (A. belladonna). It is a non-selective, competitive antagonist of the muscarinic acetylcholine receptor types M1, M2, M3, M4, and M5 (pKBs range from 8.9-9.8). Atropine increases firing of...

(+)-Pilocarpine is a muscarinic acetylcholine receptor agonist that binds to human hippocampal, pons, and submandibular gland membranes, which are endogenously enriched in the M1, M2, and M3 receptor subtypes, respectively (apparent Kis = 6, 8.2, and 6.9 µM, respectively). It induces salivary secretion in rats when...

LY2119620 is a positive allosteric modulator of the M2 and M4 muscarinic acetylcholine receptors (mAChRs). It binds to allosteric sites on M2 and M4 receptors with KB values ranging from 1.8 to 3.4 µM. LY2119620 potentiates the activity of the AChR agonist acetylcholine (ACh, Cay-23829) and the mAChR agonists...

Oxotremorine M is a non-selective muscarinic (M) acetylcholine receptor agonist that induces phosphoinositide hydrolysis in mouse ileum isolated from wild-type, M2, M3, and M2/M3 knockout mice (EC50s = 0.36, 0.52, 1.62, and 1.48 µM, respectively). It also increases M4-induced inhibition of calcium currents in...

Bethanechol is an agonist of muscarinic acetylcholine receptors with IC50 values of 1,837, 25, 631, 317, and 393 µM for M1-5, respectively, in a radioligand binding assay using CHO cells expressing the human receptors. It inhibits M2-mediated increases in cyclic AMP induced by isoproterenol (Cay-15592) in isolated...

Oxybutynin is an antagonist of muscarinic acetylcholine receptors (Kis = 5, 14.5, 3.7, 5.3, and 40 nM for human recombinant M1-5, respectively). It inhibits intracellular calcium mobilization induced by carbamoylcholine (carbachol, Cay-14486) in bladder smooth muscle and submandibular gland cells isolated from...

Dicyclomine is an M1 and M2 muscarinic acetylcholine receptor antagonist (Kis = 3.16 and 44.7 nM, respectively). It inhibits inositol phosphate accumulation induced by the non-selective acetylcholine agonist carbamoylcholine (carbachol, Cay-14486) in guinea pig cortex (Ki = 12 nM, pA2 = 7.88). Dicyclomine (10 mg/kg,...

Umeclidinium is an antagonist of muscarinic acetylcholine receptors (Kis = 0.05-0.16 nM for M1-M5 human recombinant receptors). It inhibits acetylcholine-induced activation of recombinant M3 receptors in CHO cell membranes (IC50 = 50 = 10 µM) and bronchoconstriction in mice when administered intranasally (ED50 =...