Anti-Matrix Metalloproteinase 2 (MMP-2, MMP2, MMP-II, Collagenase IV, 72kD Type IV Collagenase, Coll

Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP-2, a protein involved in the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus, detection of the enzyme is a possible early indicator of tumor activity. Positive Control: Lung, nerve, and various tumors. Applications: Suitable for use in ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot and Other applications not tested. Recommended Dilution: ELISA: 1ug/ml, Immunocytochemistry/Immunofluorescence: 1:10 -1:500, Immunohistochemistry (frozen): 5ug/ml, Immunohistochemistry (paraffin): 1:10 -1:500, Immunoprecipitation: 1:10 -1:500, Western Blot: 2-5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.