Anti-GABAA Receptor beta1 Subunit

GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS. They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: alpha1-6, beta1-4, gamma1-3, delta, epsilon, pi, theta, and rho1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by alpha1beta2gamma2 subunit-containing GABAA receptors, the major isoform found in the brain. The beta subunit of GABAA receptors interfaces with an alpha subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the gamma subunit. Phosphorylation of the beta1 subunit by PKA or PKC inhibits binding of the beta1 subunit with the clathrin adaptor protein AP2 and reduces GABAA receptor endocytosis. Mice expressing mutations in Gabrb1, which encodes the beta1 subunit isoform, have increased GABAA receptor-mediated tonic inhibition in the nucleus accumbens and increased alcohol consumption compared to wild-type mice. SNPs in GABRB1 are associated with increased impulsivity and reward sensitivity in human adolescents. Cayman's GABAA Receptor beta1 Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes the GABAA receptor beta1 subunit at approximately 55 kDa from mouse and rat samples.Synonyms: GABAA Receptor Subunit beta1, GABRB2, Gamma-aminobutyric Acid (GABA) A Receptor, beta1, Gamma-aminobutyric Acid Receptor Subunit beta1. Immunogen: Fusion protein from the cytoplasmic loop of the beta1 subunit of the rat GABAA receptor. MW: ~55 kDa. Formulation: (Request formulation change), 100 µl of affinity-purified polyclonal antibody from pooled serum. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: WB.