Anti-erbB3 (HER-3, Receptor Tyrosine-protein Kinase erbB-3, Proto-oncogene-like Protein c-ErbB-3, Ty

erbB3/HER3 is a receptor tyrosine kinase and a member of the epidermal growth factor receptor family. erbB3/HER3 binds the heregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain with a predicted molecular weight ~148kD. Signaling through the erbB3/HER3 receptor induces proliferation and differentiation in some cell types, the receptor is widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. erbB3/HER3 forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors, expression of erbB3/HER3 reported be linked to favorable outcome in bladder and ovarian cancer. erbB3/HER3 has been shown to interact with a number of proteins including EGF receptor, erbB2/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, and others. The erb3/HER3 receptor is extensively phosphorylated at residues T873 and S1123 (by cdk5), Y1199, Y1262, Y1289, and Y1328. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000, Immunohistochemistry: 1:50-1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.