Anti-CD274 (B7-h1 Protein, B7-H1, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1) (APC)

B7-H1, also known as PD-L1 and CD274, is a 50kD type I transmembrane protein belonging to the B7 family. B7-H1 contains one Ig-like C2-type domain and one Ig-like V-type domain. It is constitutively expressed in several tissues and is up-regulated on various tumors and activated dendritic cells, macrophages, and B cells. B7-H1 interacts with PD-1 on T cells to limit T cell activation and regulate immune tolerance. Within the extracellular region, mouse and human B7-H1 share 73% aa sequence identity. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Excitation: 620-650nm , Emission: 660-670nm, Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.