Anti-Cadherin E (Cadherin-1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Applications: , Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: ELISA (2) : 1:10,000 (using immobilized A431 cells) , Western Blotting: 1 - 2ug/ml (reducing or non-reducing condition)., Inhibition of E-cadherin-dependent cell-cell contact (4) : 5-10ug/ml , Immunoblotting (2) : 1 - 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume with 40% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.