Anti-ASAH1 (N-acylsphingosine amidohydrolase, Non-lysosomal ceramidase 1, acid ceramidase)

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis. Multiple isoforms of this protein are known to exist. Applications: Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, Immunohistochemistry: 2.5ug/ml, Immunofluorescence: 20ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Mouse Heart Tissue Lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.